评估 SARS-CoV-2 抗体持久性和粪便中的病毒传播：COVID-19 疫苗接种前的长期护理经验。
Evaluation of SARS-CoV-2 antibody persistence and viral spread in stool: a long-term care experience before COVID-19 vaccination.
机构信息
Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey.
Division of Medical Virology, Department of Medical Microbiology, Dokuz Eylul University Faculty of Medicine, Izmir, Turkey.
出版信息
Ir J Med Sci. 2023 Feb;192(1):263-268. doi: 10.1007/s11845-022-03095-7. Epub 2022 Jul 12.
BACKGROUND
Due to elderly residents, nursing homes/assisted living facilities were the most affected places in COVID-19 pandemic. Besides symptomatic patients, asymptomatic patients were detected during routine screening.
AIM
This study aims to determine the factors that affect antibody response and viral shedding in stool samples after natural exposure to the virus in residents and staff who recovered from COVID-19 before the vaccine was available.
METHODS
This prospective cross-sectional study was conducted at the nation's highest-capacity Residential and Nursing Home. Blood samples were collected between December 15, 2020 and January 15, 2021 from participating residents and staff for anti-SARS-CoV-2 antibody testing. Stool samples were obtained for SARS-CoV-2 PCR testing 2 months after COVID-19. The Social Sciences (SPSS) program version 15.0 was used for statistical analysis. The Mann-Whitney U test compared SARS-CoV-2 antibody concentration between two groups.
RESULTS
Four hundred sixty-four (52.3%) residents and 424 (47.7%) staff participated. Entirely 259 (29.2%) participants were anti-SARS-CoV-2 IgG (+) and 255 (28.7%) were SARS-CoV-2 PCR (+). Both antibody and PCR positivity was detected in 196 (76.9%). In PCR (-) group, 63 (10.0%) participants were SARS-CoV-2 IgG (+). Antibody titers were found highest in SARS-CoV-2 PCR (+) male residents. SARS-CoV-2 IgG titers were significantly high in SARS-CoV-2 PCR (+) and hospitalized participants regardless of age. Stool samples were obtained from 61(23.9%) participants and were found negative.
CONCLUSION
A durable SARS-CoV-2 IgG antibody response was monitored at least 9 months after the participants were diagnosed with COVID-19. SARS-CoV-2 antibody positivity was detected 76.9% in PCR (+) and 10.0% in PCR (-) participants. Knowing the duration of detectable antibodies is an important finding for developing disease prevention and public health strategies.
背景
由于老年居民的存在，养老院/辅助生活设施是 COVID-19 大流行期间受影响最大的地方。除了有症状的患者外，无症状患者也在常规筛查中被发现。
目的
本研究旨在确定在疫苗可用之前从 COVID-19 中康复的居民和工作人员在自然暴露于病毒后，影响粪便样本中抗体反应和病毒脱落的因素。
方法
这项前瞻性的横断面研究在全国容量最大的住宅和养老院进行。于 2020 年 12 月 15 日至 2021 年 1 月 15 日期间采集参与居民和工作人员的血液样本进行抗 SARS-CoV-2 抗体检测。COVID-19 后 2 个月采集粪便样本进行 SARS-CoV-2 PCR 检测。采用社会科学（SPSS）程序 15.0 进行统计分析。Mann-Whitney U 检验比较两组 SARS-CoV-2 抗体浓度。
结果
464 名（52.3%）居民和 424 名（47.7%）工作人员参与了研究。共有 259 名（29.2%）参与者抗 SARS-CoV-2 IgG（+），255 名（28.7%）为 SARS-CoV-2 PCR（+）。两者均阳性者 196 例（76.9%）。PCR（-）组中，63 名（10.0%）参与者 SARS-CoV-2 IgG（+）。PCR（+）男性居民的 SARS-CoV-2 抗体滴度最高。无论年龄大小，SARS-CoV-2 PCR（+）和住院患者的 SARS-CoV-2 IgG 滴度均显著升高。从 61 名（23.9%）参与者中获得粪便样本，结果均为阴性。
结论
至少在参与者被诊断为 COVID-19 后 9 个月监测到持续的 SARS-CoV-2 IgG 抗体反应。PCR（+）参与者中 SARS-CoV-2 抗体阳性率为 76.9%，PCR（-）参与者中 SARS-CoV-2 抗体阳性率为 10.0%。了解可检测抗体的持续时间是制定疾病预防和公共卫生策略的重要发现。
Zotero 插件