Department of Food Science and Biology Engineering, Tianjin Agricultural University, Tianjin, 300384, China.
Anal Sci. 2022 Oct;38(10):1277-1287. doi: 10.1007/s44211-022-00157-9. Epub 2022 Jul 12.
A novel capillary electrochromatography method has been developed for the simultaneous quantification of ten benzimidazole fungicides in fruits. Herein, covalent organic frameworks (COFs) and ionic liquids (ILs) were successfully introduced to prepare open-tubular capillary column to improve the loading capacity and separation performance. The parameters effecting the analytical performance including pH and concentration of running buffer, separation voltage and the addition of organic solvent were investigated systematically. Under the optimized conditions, the method allowed the baseline separation of ten benzimidazole fungicides, and showed a good linearity in the range of 3.5-200 μg kg with the detection limits between 1.0 and 2.8 μg kg. The intraday and interday precisions for recoveries were lower than 7.9% and 12.2%, respectively. Intraday and interday precisions for their retention times were lower than 3.2% and 6.6%, respectively. Satisfactory recoveries for grape, pear and orange samples at two concentrations were obtained ranging from 85.0 to 95.9% with RSDs lower than 7.8%, demonstrating the potential applications of the open-tubular capillary electrochromatography method for trace benzimidazole fungicides analysis in fruits.
一种新的毛细管电色谱法已被开发用于同时定量分析水果中的十种苯并咪唑类杀菌剂。在此，共价有机框架（COFs）和离子液体（ILs）被成功引入到制备开管毛细管柱中，以提高负载能力和分离性能。系统研究了影响分析性能的参数，包括运行缓冲液的 pH 值和浓度、分离电压以及有机溶剂的添加。在优化条件下，该方法允许十种苯并咪唑类杀菌剂基线分离，并在 3.5-200μg kg 的范围内表现出良好的线性，检测限在 1.0-2.8μg kg 之间。回收率的日内和日间精密度均低于 7.9%和 12.2%。其保留时间的日内和日间精密度均低于 3.2%和 6.6%。在两个浓度下，对葡萄、梨和橙子样品进行了回收率测定，回收率在 85.0%至 95.9%之间，RSD 低于 7.8%，证明了开管毛细管电色谱法在水果中痕量苯并咪唑类杀菌剂分析中的潜在应用。
