Liu Cuicui, Deng Qiliang, Fang Guozhen, Dang Meng, Wang Shuo
Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Food Safety & Low Carbon Manufacturing Collaborative Innovation Center, Tianjin University of Science and Technology, Tianjin 300457, China; Department of Food Science and Biology Engineering, Tianjin Agricultural University, 300384 Tianjin, China.
Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Food Safety & Low Carbon Manufacturing Collaborative Innovation Center, Tianjin University of Science and Technology, Tianjin 300457, China.
Anal Biochem. 2017 Aug 1;530:50-56. doi: 10.1016/j.ab.2017.04.014. Epub 2017 Apr 25.
Alpha-fetoprotein (AFP) is widely used as a tumor marker for the serum diagnosis of primary hepatoma. Sensitive detection of AFP level plays an important role in the early diagnosis of disease and highly reliable prediction. In this study, a novel non-competitive immunoassay (IA) based on poly(guanidinium ionic liquid) monolithic material was developed for detecting ultra trace levels of AFP in capillary electrochromatography (CEC) mode. The AFP was mixed with an excess amount of fluorescently labeled antibody. After incubation, the immunocomplex was separated from the free labeled antibody and detected by CEC coupled with laser-induced fluorescence detector. Under the optimized conditions, the developed CEC-IA performed a low detection limit of 0.05 μg L (S/N = 3) and a wide linearity ranging from 0.1 to 1000 μg L for AFP, which can be largely attributed to the high separation and enrichment efficiency of poly(guanidinium ionic liquid) monolithic material for the targets. The application of this method was demonstrated by determining AFP in human serum.
甲胎蛋白（AFP）被广泛用作原发性肝癌血清诊断的肿瘤标志物。灵敏检测AFP水平在疾病的早期诊断和高度可靠的预测中发挥着重要作用。在本研究中，基于聚（胍离子液体）整体材料开发了一种新型非竞争性免疫分析方法，用于在毛细管电色谱（CEC）模式下检测超痕量水平的AFP。将AFP与过量的荧光标记抗体混合。孵育后，将免疫复合物与游离标记抗体分离，并通过与激光诱导荧光检测器联用的CEC进行检测。在优化条件下，所开发的CEC免疫分析方法对AFP的检测限低至0.05 μg/L（S/N = 3），线性范围宽，为0.1至1000 μg/L，这在很大程度上归因于聚（胍离子液体）整体材料对目标物的高分离和富集效率。通过测定人血清中的AFP证明了该方法的应用。
