Programa de Pós-Graduação em Microbiologia Agrícola e do Ambiente, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Arch Microbiol. 2022 Jul 13;204(8):476. doi: 10.1007/s00203-022-03093-4.
Despite the worldwide use of 16S rRNA to identify bacterial species, the use of this gene does not discriminate the 750 species in the genus Streptomyces. A MLST scheme was constructed with rpoB, gyrB, recA, trpB and atpD genes to access the genomic variances in Streptomyces species evolution. We analyze the housekeeping genes in 49 Streptomyces isolates from Antarctic soil. It was used two different databases, GenBank and EzBioCloud to compare the 16S sequences. The species founded in both databases are not the same, but in both cases, a few isolates achieve the necessary high percentage to consider the identification. There is a lack of deposited sequences in the other genes, as the data in GenBank proved to be insufficient. Isolate LMA323St_9 has the potential to be studied as a novel species. Besides that, the use of housekeeping genes gives robust phylogenetic information to understand in group relationships.
尽管 16S rRNA 在全球范围内被用于鉴定细菌物种，但该基因并不能区分链霉菌属中的 750 个物种。我们构建了一个 rpoB、gyrB、recA、trpB 和 atpD 基因的 MLST 方案，以评估链霉菌物种进化中的基因组变异。我们分析了来自南极土壤的 49 株链霉菌分离株的看家基因。我们使用了两个不同的数据库，GenBank 和 EzBioCloud 来比较 16S 序列。在这两个数据库中发现的物种并不相同，但在两种情况下，一些分离株都达到了必要的高比例，足以考虑鉴定。其他基因的序列缺乏，因为 GenBank 中的数据证明是不够的。分离株 LMA323St_9 具有作为新型物种进行研究的潜力。此外，使用看家基因可以提供稳健的系统发育信息，以了解群组关系。
