Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, China.
Key Laboratory of Xinjiang Phytomedicine Resources for Ministry of Education, School of Pharmacy, Shihezi University, Shihezi, 832000, China.
Anal Bioanal Chem. 2022 Aug;414(20):6271-6280. doi: 10.1007/s00216-022-04199-w. Epub 2022 Jul 12.
Creatinine, as a significant biomarker for kidney, thyroid, and muscle dysfunction-related diseases, detection is of great important meaning. In this paper, an enzyme-nanozyme cascade sensing platform was developed for visual creatinine detection. Perovskite oxide BiFeO synthesized by a sol-gel method was applied as a nanozyme, showing excellent peroxidase-like activity. During detection, creatinine was oxidized in turn by three natural enzymes (creatinase, creatininase, and sarcosine oxidase) to produce HO, and HO was then catalyzed by the BiFeO nanozyme, resulting in the change of achromatous 3,3',5,5'-tetramethylbenzidine (TMB) into blue oxidized TMB (oxTMB). Based on this principle, visual quantification of creatinine was realized. Due to the high stability and catalytic efficiency of nanozyme, the cascade sensing platform can be used to detect creatinine in a broad range of 0.5-150 μM with a detection limit of 0.09 μM. Meanwhile, thanks to the specificity of the natural enzymes, the platform exhibited admirable selectivity for creatinine determination despite the existence of a variety of interfering substances, which were successfully adopted to measure the level of creatinine in human serums. The cascade sensing platform is expected to serve the determination of a large number of biomarkers by simply alternating the natural enzymes.
肌氨酸酐作为一种重要的生物标志物，与肾脏、甲状腺和肌肉功能障碍相关疾病的检测具有重要意义。本文开发了一种基于酶-纳米酶级联传感平台用于可视化肌氨酸酐检测。采用溶胶-凝胶法合成的钙钛矿氧化物 BiFeO 作为纳米酶，表现出优异的过氧化物酶样活性。在检测过程中，肌氨酸酐依次被三种天然酶（肌氨酸酶、肌氨酸酶和肌氨酸氧化酶）氧化生成 HO，然后 HO 被 BiFeO 纳米酶催化，导致无色 3,3',5,5'-四甲基联苯胺（TMB）变成蓝色氧化 TMB（oxTMB）。基于这一原理，实现了肌氨酸酐的可视化定量。由于纳米酶的高稳定性和催化效率，级联传感平台可以在 0.5-150 μM 的较宽范围内检测肌氨酸酐，检测限为 0.09 μM。同时，由于天然酶的特异性，该平台对肌氨酸酐的测定表现出令人钦佩的选择性，尽管存在多种干扰物质，仍能成功地用于测量人血清中的肌氨酸酐水平。该级联传感平台有望通过简单地交替使用天然酶来用于大量生物标志物的测定。
