Department of Veterinary Anatomy and Histology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (I), Aizawl, Mizoram, India.
Microsc Res Tech. 2022 Oct;85(10):3418-3430. doi: 10.1002/jemt.24197. Epub 2022 Jul 13.
This study aims to characterize the cytomorphology and ultrastructure of blood cells of native cattle of Mizoram. Twelve numbers of blood samples (10 ml) were collected from the Zobawng cattle, irrespective of sex. Blood smears were prepared and stained with different stains for cytomorphological study. The standard protocol has been followed for preparing blood samples for electron microscopy. Under a light microscope, erythrocytes of cattle were non-nucleated and round. The neutrophils were round, and the cytoplasm contained cytoplasmic granules. The eosinophils were rounded in outline with distinct cytoplasmic granules. The presence of basophils was infrequent with distinct blue color cytoplasmic granules. Small, medium, and large types of lymphocytes were recorded. The monocytes were round to oval in outline. Platelets were irregular to round. The reticulocytes were recorded occasionally, like small blue thin rods or granules. The cytoplasm and nucleus of granulocytes fluoresced greenish-yellow and red, respectively, with supravital stain. Under scanning electron microscopy (SEM), the erythrocytes appeared as biconcave discs. Different leukocytes were observed with their finger-like, plate-like, and narrow cell processes on their surface. Platelets were irregular structures. In transmission electron microscopy (TEM), the erythrocytes appeared anucleated biconcave elongated, neutrophils were roughly rounded with small cytoplasmic processes, and eosinophils were roughly circular with small cytoplasmic processes, the basophils were roughly circular with oval to elongated cytoplasmic granules, lymphocytes were roughly circular with centrally placed well-marked oval indented nucleus and some cytoplasmic processes, monocytes appeared spherical with long thick cytoplasmic processes and the non-nucleated platelets appeared roughly round to elongated. RESEARCH HIGHLIGHTS: Blood tests are a very good tool for determining animal health and diagnosing hematological illnesses. This study showed the cytomorphological and ultrastructural features of the blood cells of native cattle (Zobawng) of Mizoram state of India. The cytomorphological studies revealed that the morphological features of the blood cells of Zobawng cattle resembled to other domestic and wild animals, however, there were some differences in the cellular components. Nuclear DNA appeared orthochromatically greenish-yellow, but cytoplasmic RNA was observed to be metachromatically red under the fluorescence microscope. scanning electron microscopic (SEM) and transmission electron microscopic (TEM) results revealed that the ultrastructural features of the blood cells of Zobawng cattle contain certain cellular morphological differences as compared to the other domestic and wild animals.
本研究旨在描述米佐拉姆邦本地牛的血细胞的细胞形态学和超微结构。从 Zobawng 牛身上采集了 12 份（每份 10ml）血液样本，不分性别。制备血涂片并用不同的染色剂进行染色以进行细胞形态学研究。为制备电子显微镜血液样本，遵循了标准方案。在光镜下，牛的红细胞无核且呈圆形。中性粒细胞呈圆形，细胞质中含有细胞质颗粒。嗜酸性粒细胞呈圆形，轮廓清晰，细胞质颗粒明显。嗜碱性粒细胞的存在频率较低，细胞质颗粒呈明显的蓝色。记录了小、中、大三种类型的淋巴细胞。单核细胞呈圆形到椭圆形。血小板形状不规则，呈圆形。偶尔会记录到网织红细胞，如小的蓝色细棒或颗粒。用活体染色，粒细胞的细胞质和细胞核分别发出黄绿色和红色荧光。在扫描电子显微镜（SEM）下，红细胞呈双凹盘状。在表面观察到不同的白细胞，具有指状、板状和窄的细胞突起。血小板为不规则结构。在透射电子显微镜（TEM）下，红细胞呈无核双凹拉长形，中性粒细胞大致呈圆形，有小的细胞质突起，嗜酸性粒细胞大致呈圆形，有小的细胞质突起，嗜碱性粒细胞大致呈圆形，有椭圆形到拉长形的细胞质颗粒，淋巴细胞大致呈圆形，中央有明显的椭圆形凹陷核和一些细胞质突起，单核细胞呈球形，有长而粗的细胞质突起，无核血小板大致呈圆形到拉长形。研究亮点：血液测试是确定动物健康和诊断血液疾病的非常好的工具。本研究显示了印度米佐拉姆邦本地牛（Zobawng）的血细胞的细胞形态学和超微结构特征。细胞形态学研究表明，Zobawng 牛的血细胞形态特征与其他家畜和野生动物相似，但细胞成分存在一些差异。核 DNA 在荧光显微镜下呈现出典型的绿黄色，而细胞质 RNA 呈现出典型的红色。扫描电子显微镜（SEM）和透射电子显微镜（TEM）的结果表明，与其他家畜和野生动物相比，Zobawng 牛的血细胞的超微结构特征包含某些细胞形态学差异。
