Choudhary Om Prakash, Kalita Pranab Chandra, Kalita Arup, Doley Probal Jyoti, Sarkar Rupan, Eregowda Chethan Gollahalli, Choudhary Priyanka
Department of Veterinary Anatomy and Histology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (I), Aizawl, Mizoram, India.
Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (I), Aizawl, Mizoram, India.
Vet Clin Pathol. 2023 Sep;52(3):379-385. doi: 10.1111/vcp.13213. Epub 2023 May 11.
Ultrastructural information regarding the peripheral blood cells of local (Zovawk) pigs from Mizoram, India, is not available in the scientific literature.
The present study was designed to reveal the fine structural details of the blood cells from these local pigs using a transmission electron microscope (TEM).
Blood samples were collected from 12 healthy Zovawk pigs of either sex and processed according to a standard protocol. Processed blood samples were then sent to the All India Institute of Medical Sciences, New Delhi, for further processing and imaging under TEM. Different types of blood cells were viewed under TEM, and different characteristics of these cells were assessed.
In the present study, erythrocytes are elongated, biconcave, and nucleated without cytoplasmic organelles. Neutrophils are round with 2-5 lobed nuclei surrounded by cytoplasm with an indistinct bilayered nuclear membrane. The cytoplasm is packed with membrane bound round, oval, and elongated cytoplasmic granules. Eosinophils are round to oval with 2-3 lobed nuclei with distinct nuclear membranes. Basophils are spherical and contained small, medium, and large electron-dense granules. Lymphocytes are small, medium, and large and contained all cellular components. Monocytes are irregularly spherical with slight nuclear indentations. The platelets are elongated, oval, or rounded, with a few pseudopods at the cell surface.
From the present study, we can conclude that the ultrastructural morphology of blood cells from Zovawk pigs resembles those of other domestic animals. However, a few differences have been observed.
关于印度米佐拉姆邦当地（佐沃克）猪外周血细胞的超微结构信息在科学文献中尚无记载。
本研究旨在使用透射电子显微镜（TEM）揭示这些当地猪血细胞的精细结构细节。
从12头健康的佐沃克猪（雌雄不限）采集血样，并按照标准方案进行处理。然后将处理后的血样送往新德里的全印度医学科学研究所进行进一步处理和在TEM下成像。在TEM下观察不同类型的血细胞，并评估这些细胞的不同特征。
在本研究中，红细胞呈细长形、双凹形且有核，无细胞质细胞器。中性粒细胞呈圆形，有2 - 5叶核，被细胞质包围，核膜不明显，呈双层。细胞质中充满了膜结合的圆形、椭圆形和细长形细胞质颗粒。嗜酸性粒细胞呈圆形至椭圆形，有2 - 3叶核，核膜明显。嗜碱性粒细胞呈球形，含有小、中、大的电子致密颗粒。淋巴细胞有小、中、大三种，包含所有细胞成分。单核细胞呈不规则球形，核有轻微凹陷。血小板呈细长形、椭圆形或圆形，细胞表面有一些伪足。
从本研究中，我们可以得出结论，佐沃克猪血细胞的超微结构形态与其他家畜相似。然而，也观察到了一些差异。
