hsa_circ_000 1429 吸附 miR-205 调控 KDM4A 促进乳腺癌转移的机制研究。

Investigation of the Mechanism of hsa_circ_000 1429 Adsorbed miR-205 to Regulate KDM4A and Promote Breast Cancer Metastasis.

机构信息

Department of Breast Cancer Surgery, Harbin Medical University Cancer Hospital, Harbin 150081, China.

出版信息

Contrast Media Mol Imaging. 2022 Jun 21;2022:4657952. doi: 10.1155/2022/4657952. eCollection 2022.

This study investigates the mechanism of hsa_circ_0001429 adsorbing miR-205 and regulating the expression of KDM4A to promote breast cancer metastasis and its mechanism. Mammary epithelial cells MCF-10A and human breast cancer cell lines BT474, SKBr-3, ZR-75-30, and MCF7 are cultured, and the mRNA expressions of hsa_circ_000 1429, miR-205, and KDM4A are detected by qRT-PCR; hsa_circ_000 1429 binds to miR-205, and miR-205 targets KDM4A. RIP verifies that hsa_circ_000 1429 binds to AGO2; RNA pull down results prove that hsa_circ_000 1429 binds to miR-205; MTT detects cell proliferation; transwell assay detects cell migration and invasion ability; flow cytometry detects cell apoptosis rate. The expressions of KDM4A, migration, and invasion-related factors, N-cadherin and MMP-9 protein, are detected by blot. hsa_circ_000 1429 may upregulate the KDM4A gene by adsorbing miR-205. Therefore, it will promote the proliferation, migration, and invasion of breast cancer cells and inhibit their apoptosis.

本研究旨在探讨 hsa_circ_0001429 吸附 miR-205 并调控 KDM4A 表达以促进乳腺癌转移的机制及其作用机制。培养乳腺上皮细胞 MCF-10A 和人乳腺癌细胞系 BT474、SKBr-3、ZR-75-30 和 MCF7，采用 qRT-PCR 检测 hsa_circ_0001429、miR-205 和 KDM4A 的 mRNA 表达；hsa_circ_0001429 与 miR-205 结合，miR-205 靶向 KDM4A。RIP 验证 hsa_circ_0001429 与 AGO2 结合；RNA 下拉结果证明 hsa_circ_0001429 与 miR-205 结合；MTT 检测细胞增殖；transwell 检测细胞迁移和侵袭能力；流式细胞术检测细胞凋亡率。通过印迹法检测 KDM4A 基因、迁移和侵袭相关因子 N-钙黏蛋白和 MMP-9 蛋白的表达。hsa_circ_0001429 可能通过吸附 miR-205 而上调 KDM4A 基因。因此，它将促进乳腺癌细胞的增殖、迁移和侵袭，并抑制其凋亡。