van Rooijen N, Kors N, Claassen E
Immunol Lett. 1987 Apr;14(4):313-9. doi: 10.1016/0165-2478(87)90011-3.
Mice were immunized i.v. with 2,4,6-trinitrophenyl (TNP)-carrier conjugates and received lipopolysaccharide (LPS) before, simultaneously with or after these antigens, in order to study the effects of LPS on the in situ development of anti-TNP antibody forming cells in the spleen. LPS given before, simultaneously with or shortly after the thymus dependent (TD) antigen TNP-keyhole limpet haemocyanin (KLH) suppressed the development of anti-TNP antibody forming cells in the spleen, but serum IgM-anti-TNP titres were not reduced, suggesting a selective inhibiting effect of LPS on the local immune response in the spleen. Splenic responses to thymus independent (TI) antigens TNP-Ficoll and TNP-LPS were not reduced when these antigens were administered one day after LPS. LPS given 4 days after TNP-KLH and 1 day before killing of the animals had induced severe alterations in splenic histology, but we found no marked influence on numbers and distribution pattern of anti-TNP forming cells. Results indicate that the LPS induced suppression of the immune response to TD antigens in the spleen is not caused by a direct effect of LPS on antigen reactive B-lymphocytes or on the anti-TNP antibody forming cells themselves. Possible explanations for the LPS-induced immunosuppression are discussed.
用2,4,6-三硝基苯基（TNP）-载体缀合物对小鼠进行静脉免疫，并在这些抗原之前、同时或之后给予脂多糖（LPS），以研究LPS对脾脏中抗TNP抗体形成细胞原位发育的影响。在胸腺依赖性（TD）抗原TNP-钥孔戚血蓝蛋白（KLH）之前、同时或之后不久给予LPS，可抑制脾脏中抗TNP抗体形成细胞的发育，但血清IgM-抗TNP滴度未降低，这表明LPS对脾脏局部免疫反应具有选择性抑制作用。当在LPS给药一天后给予胸腺非依赖性（TI）抗原TNP-菲可和TNP-LPS时，脾脏对它们的反应并未降低。在TNP-KLH给药4天后且在处死动物前1天给予LPS，可引起脾脏组织学的严重改变，但我们未发现其对抗TNP形成细胞的数量和分布模式有明显影响。结果表明，LPS诱导的脾脏对TD抗原免疫反应的抑制不是由LPS对抗原反应性B淋巴细胞或抗TNP抗体形成细胞本身的直接作用引起的。本文讨论了LPS诱导免疫抑制的可能解释。
