School of Pharmacy, Anhui University of Chinese Medicine, Hefei, China.
Anhui Genuine Chinese Medicinal Materials Quality Improvement Collaborative Innovation Center, Hefei, China.
J Pharm Pharmacol. 2022 Sep 1;74(9):1230-1240. doi: 10.1093/jpp/rgac012.
This study aims to compare the fingerprint and the content of the three components of sweated and non-sweated Salvia miltiorrhiza alcoholic extracts (SSAE and NSAE). It also aims to investigate the difference in protective effects of SSAE and NSAE on myocardial ischaemia-reperfusion injury (MIRI).
The fingerprints of SSAE and NSAE were established by HPLC with a UV detector to identify the common peaks and detect the content of the three major components (cryptotanshinone, tanshinone I and tanshinone IIA). The protective effects of SSAE and NSAE were compared with MIRI rat model after orally administered SSAE and NSAE (2 g/kg of raw drug) for 7 days. The ST segment, PR and QT interval changes and the infarct size were assessed in the rat hearts. Moreover, the activity of aspartate transaminase (AST), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and the level of cardiac troponin I (cTn I) in serum as well as the cardiac H&E staining were evaluated.
The results showed that the fingerprints of SSAE and NSAE were similar, and cluster analysis showed that the sweating methods had effects on the alcoholic extracts. The content determination showed that sweating could increase the total content of cryptotanshinone, tanshinone I and tanshinone IIA of S. miltiorrhiza. The results of electrocardiograms (ECG) showed that SSAE could make the ST segment drop more obviously, PR and QT intervals become shorter, and the size of the infarct much smaller. Compared with NSAE, SSAE had more significant effects on the enzymatic activity of AST, LDH and the level of cTn I in serum. The H&E staining showed that both SSAE and NSAE could reduce the degree of heart damage.
The present investigation results demonstrated that sweating increased the content of tanshinone components in S. miltiorrhiza alcoholic extracts, and SSAE had a better protective effect on MIRI.
本研究旨在比较丹参酒提物（SSAE 和 NSAE）的指纹图谱和三个成分（隐丹参酮、丹参酮 I 和丹参酮 IIA）的含量，并探讨 SSAE 和 NSAE 对心肌缺血再灌注损伤（MIRI）的保护作用差异。
采用高效液相色谱法（HPLC）结合紫外检测器建立 SSAE 和 NSAE 的指纹图谱，鉴定共有峰并检测三个主要成分的含量。将 SSAE 和 NSAE 口服给药（生药 2 g/kg）7 天后，建立 MIRI 大鼠模型，比较其对 MIRI 的保护作用。评估大鼠心脏的 ST 段、PR 和 QT 间隔变化和梗死面积，同时评估血清中天冬氨酸转氨酶（AST）、乳酸脱氢酶（LDH）、超氧化物歧化酶（SOD）活性和心肌肌钙蛋白 I（cTn I）水平，以及心脏 H&E 染色。
结果表明，SSAE 和 NSAE 的指纹图谱相似，聚类分析表明，发汗方法对酒提物有影响。含量测定表明，发汗可增加丹参总隐丹参酮、丹参酮 I 和丹参酮 IIA 的含量。心电图（ECG）结果表明，SSAE 可使 ST 段下降更明显，PR 和 QT 间隔缩短，梗死面积更小。与 NSAE 相比，SSAE 对血清中 AST、LDH 酶活性和 cTn I 水平的影响更为显著。H&E 染色表明，SSAE 和 NSAE 均可减轻心脏损伤程度。
本研究结果表明，发汗可增加丹参酒提物中丹参酮成分的含量，SSAE 对 MIRI 具有更好的保护作用。
