中国百合属植物中一种新型虹彩病毒的全长基因组序列。

The full-length genome sequence of a novel amalgavirus in Lilium spp. in China.

机构信息

Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.

Key Laboratory of Horticultural Plant Biology (Huazhong Agricultural University), Ministry of Education, Wuhan, China.

出版信息

Arch Virol. 2022 Oct;167(10):2103-2107. doi: 10.1007/s00705-022-05523-6. Epub 2022 Jul 14.

DOI:10.1007/s00705-022-05523-6

PMID:35834002

Abstract

We report for the first time the complete genome sequence of a novel amalgavirus, tentatively designated as 'lily amalgavirus 1' (LAV-1), isolated from Lilium spp. in China. LAV-1 is a 3448-nt double-stranded RNA virus that encodes two putative proteins. Open reading frame 1 (ORF1) encodes a 394-aa protein with unknown function. ORF2 encodes a putative RNA-dependent RNA polymerase (RdRp) of 895 aa. The two ORFs putatively encode a '1 + 2' fusion protein generated by a '+1' programmed ribosomal frameshift (PRF). BLASTp analysis revealed that the complete genome sequence of LAV-1 shares 48.23-59.80% sequence identity (query sequence coverage > 77%) with those of members of the genus Amalgavirus, with the highest nucleotide sequence identity of 59.80% with that of Allium cepa amalgavirus 1 (query sequence coverage, 87%). The genome structure, phylogenetic relationships, and sequence similarities to other plant amalgaviruses suggest that LAV-1 is a new member of the genus Amalgavirus.

摘要

我们首次报道了一种新型虹彩病毒的全基因组序列，暂命名为“百合虹彩病毒 1 型”（LAV-1），该病毒从中国的百合属植物中分离得到。LAV-1 是一种 3448 个核苷酸的双链 RNA 病毒，编码两个假定的蛋白。开放阅读框 1（ORF1）编码一个 394 个氨基酸的未知功能蛋白。ORF2 编码一个推定的 RNA 依赖性 RNA 聚合酶（RdRp），由 895 个氨基酸组成。这两个 ORF 假定编码一个通过“+1”程序性核糖体移码（PRF）产生的“1+2”融合蛋白。BLASTp 分析表明，LAV-1 的全基因组序列与虹彩病毒属的成员具有 48.23-59.80%的序列同一性（查询序列覆盖率>77%），与 Allium cepa amalgavirus 1 的核苷酸序列同一性最高，为 59.80%（查询序列覆盖率为 87%）。基因组结构、系统发育关系和与其他植物虹彩病毒的序列相似性表明，LAV-1 是虹彩病毒属的一个新成员。