来自立枯丝核菌AG-3 PT菌株3P-2-2的一种新型镰孢菌病毒的全基因组序列

Complete genome sequence of a novel fusarivirus from Rhizoctonia solani AG-3 PT strain 3P-2-2.

作者信息

Sun Aili, Luo Li, Zhao Zifang, Kang Lijuan, Li Chengyun, Dong Wenhan, Yang Genhua

机构信息

State Key Laboratory for Protection and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, Yunnan, China.

Technology Department, Yunnan Agricultural University, Kunming, 650201, Yunnan, China.

出版信息

Arch Virol. 2023 Jan 27;168(2):75. doi: 10.1007/s00705-022-05673-7.

DOI:10.1007/s00705-022-05673-7

PMID:36707487

Abstract

Here, we describe a novel mycovirus, tentatively designated as "Rhizoctonia solani fusarivirus 6" (RsFV6), which was discovered in Rhizoctonia solani AG-3 PT strain 3P-2-2. The virus has a single-stranded positive-sense RNA (+ssRNA) genome of 6141 nucleotides containing two open reading frames (ORFs) and a poly(A) tail. ORF1 encodes a large polypeptide of 1,862 amino acids (aa) with conserved RNA-dependent RNA polymerase (RdRp) and helicase (Hel) domains. ORF2 encodes a putative 167-aa protein of unknown function. BLASTp searches revealed that the ORF1-encoded polypeptide showed the highest sequence similarity (70.67% identity) to that of Rhizoctonia solani fusarivirus 3 (RsFV3), which was isolated from Rhizoctonia solani AG-2-2LP. Multiple sequence alignments and phylogenetic analysis based on RdRp and Hel sequences indicated that RsFV6 could be a novel member of the genus Alphafusarivirus family Fusariviridae.

摘要

在此，我们描述了一种新型的真菌病毒，暂定名为“立枯丝核菌镰刀菌病毒6”（RsFV6），它是在立枯丝核菌AG-3 PT菌株3P-2-2中发现的。该病毒具有一个6141个核苷酸的单链正义RNA（+ssRNA）基因组，包含两个开放阅读框（ORF）和一个聚腺苷酸尾。ORF1编码一个由1862个氨基酸（aa）组成的大的多肽，具有保守的RNA依赖性RNA聚合酶（RdRp）和解旋酶（Hel）结构域。ORF2编码一个功能未知的推定的167个氨基酸的蛋白质。BLASTp搜索显示，ORF1编码的多肽与从立枯丝核菌AG-2-2LP中分离出的立枯丝核菌镰刀菌病毒3（RsFV3）的多肽具有最高的序列相似性（70.67%的同一性）。基于RdRp和Hel序列的多序列比对和系统发育分析表明，RsFV6可能是镰刀菌病毒科甲型镰刀菌病毒属的一个新成员。