Obtaining Osmo-resistant Mutants in Nitrogen-Fixing Bacteria Isolated from Saline Soils.

Annually, about, more than 7% of the Earth's land area becomes inappropriate for agriculture subsequently of salinization and desertification. Biofertilizers based on halophilic nitrogen-fixing bacteria can restore saline soils and stimulate plant growth, having a positive effect on germination, development of stems and roots, and fruiting. The aim of this work was to obtain osmo-resistant (Osm-r) nitrogen-fixing mutants isolated from saline soils of Armenia and selection of the best ones. To achieve this goal, we have obtained a collection of Osm-r strains based on soil nitrogen-fixing bacteria without the use of genetically modified technologies, which is an innovation in sphere of soil microbiology, and, especially, in nitrogen-fixing microorganisms. These mutants were obtained on the basis of Agrobacterium sp. Y-2 and Agrobacterium sp. M-1 nitrogen-fixing strains, both spontaneously and induced. Four strains with the higher nitrogen-fixing ability, which kept their vital activity in an environment with a high concentration of salts, were selected from collection of mutants. Selected strains in the future can become the basis for creating a new, effective, environmentally friendly biofertilizer for saline soils because they are plasmidless and have the highest priority for intensive use in agriculture.