Busnach G, Dal Col A, Perrino M L, Brando B, Brunati C, Minetti L
Int J Artif Organs. 1987 Mar;10(2):121-8.
Cascade filtration (CF) has been performed in 67 on-line procedures in 9 normolipidemic patients with paraproteinemic disorders. A modified dead-end technique has been employed, with high flow rate recirculating plasma on the plasma fractionation filter (QD recycled CF), and an albumin-rich, globulin-poor filtrate was reinfused into the patient. Postprocedure recoveries were 81 +/- 15% for albumin, 55 +/- 23% for IgM and 48% for cryocrit, with an increase in A/G ratio from 1.8 to 2.1. An improvement was observed also in antiatherogenic/atherogenic lipoproteins ratio, suggesting a possible use of this technique in the treatment of familial hypercholesterolemia. Plasma primary separation was obtained by centrifugation or by filtration, and no significant differences were observed on subsequent protein fractionation process. An albumin priming of the plasmafractionation circuit accounted for an additional 13% saving in postprocedure level. Different surface area secondary filters have been employed: with larger surfaces, larger volumes were processed without any increase in the waste volume and with reduced need for washouts, but with an additional loss of small molecules possibly due to entrapping onto the membrane.
对9例患有副蛋白血症的血脂正常患者进行了67次串联过滤（CF）在线操作。采用了改良的死端技术，在血浆分离滤器上以高流速循环血浆（QD循环CF），然后将富含白蛋白、球蛋白含量低的滤液回输到患者体内。术后白蛋白回收率为81±15%，IgM为55±23%，冷沉淀为48%，A/G比值从1.8增加到2.1。抗动脉粥样硬化/致动脉粥样硬化脂蛋白比值也有所改善，表明该技术可能用于治疗家族性高胆固醇血症。血浆初次分离通过离心或过滤获得，在随后的蛋白质分离过程中未观察到显著差异。血浆分离回路的白蛋白预充使术后水平额外节省了13%。使用了不同表面积的二级滤器：表面积越大，处理的体积越大，废液体积没有增加，冲洗需求减少，但可能由于膜截留导致小分子进一步损失。
