Horváth T, Karge E, Jávor T, Klinger W
Int J Clin Pharmacol Ther Toxicol. 1987 Apr;25(4):201-3.
The cytochrome P-450 system is a sensitive indicator in different forms of experimental liver diseases in the rat. Both CCl4 and ethanol produce a liver damage by free radical mechanisms causing a lesion of liver cell endoplasmic reticulum. Protective effects of antioxidants on CCl4 or ethanol induced liver damage were investigated on this basis in adult Wistar rats. The strongest protective effect in CCl4 treated animals was shown to be exerted by allopurinol. The other three antioxidants (cyanidanol, MTDQ monomer and MTDQ dimer) could not prevent the CCl4 induced changes, measured by liver/body mass quotient, P-450 concentration, ethylmorphine N-demethylation and ethoxycoumarin O-deethylation. Ethanol in the given doses did not produce any distinct effect on the test parameters. The most sensitive parameter was the ethylmorphine N-demethylation. In conclusion CCl4 intoxication is a suitable model for studying radical initiated liver injuries. The mechanisms of the hepatoprotective effects of the four substances are different.
细胞色素P-450系统是大鼠不同形式实验性肝病的敏感指标。四氯化碳（CCl4）和乙醇均通过自由基机制导致肝损伤，引起肝细胞内质网损伤。在此基础上，研究了抗氧化剂对CCl4或乙醇诱导的肝损伤的保护作用，实验对象为成年Wistar大鼠。结果表明，别嘌呤醇对CCl4处理的动物具有最强的保护作用。通过肝/体重比、P-450浓度、N-脱甲基乙基吗啡和O-脱乙基乙氧香豆素测定，其他三种抗氧化剂（花青定、MTDQ单体和MTDQ二聚体）不能预防CCl4诱导的变化。给定剂量的乙醇对测试参数没有产生任何明显影响。最敏感的参数是N-脱甲基乙基吗啡。总之，CCl4中毒是研究自由基引发肝损伤的合适模型。这四种物质的肝保护作用机制不同。
