School of Public Health, Jilin University, Changchun 130021, China; Jilin Center for Disease Prevention and Control, Changchun 130062, Changchun, China.
School of Public Health, Jilin University, Changchun 130021, China.
Int J Food Microbiol. 2022 Sep 16;377:109825. doi: 10.1016/j.ijfoodmicro.2022.109825. Epub 2022 Jul 8.
Transmission and outbreaks of Staphylococcus aureus among retail food highlights the need to comprehensive analysis the molecular characteristic of Staphylococcus aureus in foods. However, the information about Staphylococcus aureus in north China is limited. In this study, 97 and 28 S. aureus strains were isolated for analysis from 4262 samples of retail food and 61 samples food outbreaks with prevalence rate 2.28 % and 45.9 %, respectively in Jilin, China from 2014 to 2018. This study aimed to investigate the prevalence of S. aureus isolates and characterize by antimicrobial resistance testing, virulence profiles, spa typing, and multilocus sequence typing (MLST) analysis. 60 % (75/125) of the isolates contained at least enterotoxin genes including classic and new SEs genes as following: sea (40/125,32 %), see (36/125,28.8 %), sec (29/125,23.2 %), sell (29/125,23.2 %), seb (25/125,20 %), seh (22/125,17.6 %), sed (6/125,4.8 %), selq (6/125,4.8 %), and selk (6/125,4.8 %). In antimicrobial susceptibility tests, 59.2 % of the isolates (74/125) were considered as multi-drug-resistant isolates and four MRSA strains were all found with high multi-drug-resistance. Phenotype resistance to penicillin (94.4 %), erythromycin (84.2 %), clindamycin (63.9 %), and tetracycline (47.2 %) was observed which was corresponding with genotype resistance. The strains were classified to twenty-two sequence types (STs), fourteen clonal complexes (CCs), and forty-seven spa types. The predominant ST and spa types were ST1(22/125,17.6 %), ST25(20/125,16.00 %), ST398 (14/125,11.2 %) and t127 (20/125,16 %), t078 (14/125,11.2 %), t803 (7/125,5.6 %). The wgSNP analysis of these isolates in food represents showed close relatedness with food outbreaks which pose a potential health risk for consumers and warrants further attention.
从 2014 年至 2018 年，在中国吉林，本研究从 4262 份零售食品和 61 份食品暴发样本中分别分离出 97 株和 28 株金黄色葡萄球菌，流行率分别为 2.28%和 45.9%。本研究旨在调查金黄色葡萄球菌分离株的流行情况，并通过药敏试验、毒力谱、spa 分型和多位点序列分型（MLST）分析进行特征分析。60%（75/125）的分离株至少含有经典和新型 SE 基因的肠毒素基因，如下所示：sea（40/125，32%）、see（36/125，28.8%）、sec（29/125，23.2%）、sell（29/125，23.2%）、seb（25/125，20%）、seh（22/125，17.6%）、sed（6/125，4.8%）、selq（6/125，4.8%）和 selk（6/125，4.8%）。在药敏试验中，59.2%（74/125）的分离株被认为是多药耐药分离株，4 株耐甲氧西林金黄色葡萄球菌（MRSA）均发现高度多药耐药。表型对青霉素（94.4%）、红霉素（84.2%）、克林霉素（63.9%）和四环素（47.2%）的耐药性与基因型耐药性相对应。菌株被分为 22 种序列型（STs）、14 种克隆复合体（CCs）和 47 种 spa 型。主要的 ST 和 spa 型为 ST1（22/125，17.6%）、ST25（20/125，16.00%）、ST398（14/125，11.2%）和 t127（20/125，16%）、t078（14/125，11.2%）、t803（7/125，5.6%）。食品中这些分离株的 wgSNP 分析表明与食品暴发密切相关，对消费者构成潜在健康风险，值得进一步关注。
