Transcriptomic and Biochemical Analysis of the Antimicrobial Mechanism of Lipopeptide Iturin W against .

is one of the most serious pathogens threatening food safety and public health. We have previously showed that iturin W exhibited obvious antifungal activity on plant pathogens. In the present study, we found iturin W, especially C iturin W, showed strong antimicrobial activity against , and the antimicrobial mechanism of C iturin W was further investigated by transcriptomic analysis and a related biochemical experiment. The results showed that C iturin W can reduce the expression levels of genes associated with the reactive oxygen species (ROS) scavenging enzyme and genes involved in arginine biosynthesis, thus leading to the increase in ROS levels of . Furthermore, C iturin W can also interfere with proton dynamics, which is crucial for cells to regulate various biological possesses. Therefore, ROS accumulation and change in proton motive force are import ways for C iturin W to exert the antimicrobial activity. In addition, C iturin W can also reduce the expression levels of genes related to virulence factors and decrease the production of enterotoxins and hemolysins in , indicating that C iturin W has a good potential in food and pharmaceutical fields to reduce the harm caused by in the future.