Laboratorio de Toxicología Ambiental, Facultad de Química, Universidad Autónoma del Estado de México, Paseo Colón intersección Paseo Tollocan, Colonia Residencial Colón, 50120 Toluca, Estado de México, Mexico.
Laboratorio de Toxicología Ambiental, Facultad de Química, Universidad Autónoma del Estado de México, Paseo Colón intersección Paseo Tollocan, Colonia Residencial Colón, 50120 Toluca, Estado de México, Mexico.
Environ Toxicol Pharmacol. 2022 Aug;94:103925. doi: 10.1016/j.etap.2022.103925. Epub 2022 Jul 12.
This study assessed the effects of Bisphenol A in embryonic stages of zebrafish, applying an IBR multi-biomarker approach that included alterations in growth and oxidative status and relates it with the expression of Nrf1, Nrf2, Wnt3a, Wnt8a, COX-2, Qdpra, and DKK1 genes. For this purpose, we exposed zebrafish embryos to eight environmentally relevant concentrations of BPA (220, 380, 540, 700, 860, 1180, 1340, and 1500 ng L) until 96 h post-fertilization. Our results show that BPA induces several malformations in embryos (developmental delay, hypopigmentation, tail malformations, and on), leading to their death. The LC, EC of malformations, and teratogenic index (TI) were 1234.60 ng L, 987.77 ng L, and 1.25, respectively; thus, this emerging contaminant is teratogenic. Regarding oxidative stress and gene expression, we demonstrated BPA altered oxidative status and the gene expression in embryos of Danio rerio.
本研究评估了双酚 A 在斑马鱼胚胎发育阶段的影响，应用了 IBR 多生物标志物方法，包括生长和氧化状态的改变，并将其与 Nrf1、Nrf2、Wnt3a、Wnt8a、COX-2、Qdpra 和 DKK1 基因的表达相关联。为此，我们将斑马鱼胚胎暴露于 8 种环境相关浓度的 BPA（220、380、540、700、860、1180、1340 和 1500ng/L）中，直到受精后 96 小时。我们的结果表明，BPA 可诱导胚胎出现多种畸形（发育迟缓、色素减退、尾巴畸形等），导致其死亡。LC、畸形 EC 和致畸指数（TI）分别为 1234.60ng/L、987.77ng/L 和 1.25；因此，这种新兴污染物具有致畸性。关于氧化应激和基因表达，我们证明了 BPA 改变了斑马鱼胚胎的氧化状态和基因表达。
