Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, Doornfontein, South Africa.
Department of Complementary Medicine, Faculty of Health Sciences, University of Johannesburg, Doornfontein, South Africa.
Homeopathy. 2023 Feb;112(1):50-56. doi: 10.1055/s-0042-1747683. Epub 2022 Jul 14.
Cervical cancer (CC) is the second leading cancer in women and is the most common in those aged 15 to 44 years. Medicinal plant extracts have been used as homeopathic preparations for health benefits. () used to treat disorders of the female genital tract and produces cytotoxic effects. However, the use of homeopathically prepared in combination with low level laser therapy has not previously been explored.
The study aims to investigate the in-vitro effects of homeopathically prepared alone and in combination as a potential photosensitizer with Low-level laser irradiation (LLLI) at fluencies of 5, 10, and 15 J/cm.
HeLa CC cells were treated with (D3, D6, and 30cH homeopathic preparations). Cells were then treated with IC and 680 nm laser diode at 5, 10, and 15 J/cm fluencies, and the results compared with untreated control cells. Trypan blue viability, lactate dehydrogenase (LDH) cytotoxicity, and adenosine triphosphate (ATP) proliferation assays were used to analyze the cellular dose-responses along with inverted microscopy, Hoechst staining and Annexin-V/PI staining.
D3 alone demonstrated an ability to reduce cellular viability to 59% and also to reduce ATP levels. The subsequent combined treatment protocol of D3 with all fluencies of laser demonstrated an increase in cellular ATP and increased LDH levels compared with the control.
The increased ATP and LDH levels observed in the combined treatment protocol of 680 nm laser and D3 at fluencies of 5, 10 and 15 J/cm, show that the Warburg effect might have been induced in the CC cells - an increase in glucose uptake and the preferential production of lactate, even in the presence of oxygen. More research, including work on other cell lines, needs to be conducted to identify if and perhaps a different wavelength of laser irradiation could have potential in inducing cell death in cancer cells.
宫颈癌（CC）是女性中的第二大癌症，在 15 至 44 岁年龄组中最为常见。药用植物提取物已被用作顺势疗法制剂以促进健康。（）曾被用于治疗女性生殖道疾病，并产生细胞毒性作用。然而，以前尚未探索过顺势疗法制备的与低水平激光治疗联合使用的情况。
本研究旨在研究单独使用和作为潜在光敏剂与低水平激光照射（LLLI）联合使用的顺势疗法制备的（D3、D6 和 30cH 顺势疗法制剂）的体外效应，激光剂量为 5、10 和 15 J/cm。
用（D3、D6 和 30cH 顺势疗法制剂）处理 HeLa CC 细胞。然后用 680nm 激光二极管在 5、10 和 15 J/cm 剂量下处理细胞，并将结果与未处理的对照细胞进行比较。使用台盼蓝活力、乳酸脱氢酶（LDH）细胞毒性和三磷酸腺苷（ATP）增殖测定法分析细胞剂量反应，以及倒置显微镜、Hoechst 染色和 Annexin-V/PI 染色。
D3 单独处理可将细胞活力降低至 59%，并降低 ATP 水平。随后用激光所有剂量进行的联合处理方案显示，与对照相比，细胞内 ATP 增加和 LDH 水平升高。
在 680nm 激光和 D3 的联合处理方案中观察到的 ATP 和 LDH 水平增加表明，即使在存在氧气的情况下，CC 细胞中的瓦博格效应也可能被诱导-葡萄糖摄取增加和乳酸的优先产生。需要进行更多的研究，包括对其他细胞系的研究，以确定 和也许不同波长的激光照射是否有可能在诱导癌细胞死亡方面具有潜力。
