蔓越橘树皮提取物与 680nm 激光辐照联合诱导乳腺癌细胞死亡。

Combined Effect of Vaccinium nilgiriensis Bark Extract and 680nm Laser Irradiation in Inducing Breast Cancer Cell Death.

机构信息

Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, South Africa.

Bioprospecting Laboratory, Department of Botany, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India.

出版信息

Anticancer Agents Med Chem. 2021;21(2):207-213. doi: 10.2174/1871520620666200410082302.

DOI:10.2174/1871520620666200410082302

PMID:32275491

Abstract

BACKGROUND

Cancer refers to a collection of diseases where cells begin to multiply uncontrollably. Breast cancer is the most predominant malignancy in women. Herbal medicine is one of the important health care systems in most developing countries. Many studies have shown that naturally occurring compounds may support the prevention and treatment of various diseases, including cancer. Some of the plant extracts and isolated compounds show photosensitizing activities and reduce cell proliferation whereas some have revealed photoprotective effects.

OBJECTIVES

The biological properties and medicinal uses of extracts and bioactive compounds from V. nilgiriensis have not been investigated. This study aims to evaluate the cytotoxic effects of V. nilgiriensis in combination with 680nm laser irradiation on MCF-7 breast cancer cells.

METHODS

The inverted microscopy, ATP and LDH assay were used to analyze the cellular morphology, proliferation, cytotoxicity respectively after the treatment with V. nilgiriensis bark extract. The diode laser of wavelength 680nm and 15 J/cm2 fluency has been used for laser irradiation. The activity of apoptotic proteins was studied using ELISA and nuclear damage by Hoechst staining.

RESULTS

The exposure of V. nilgiriensis extracts with laser irradiation at 680nm increases the cytotoxicity and decreases the proliferation of MCF-7 cells. The results of the Hoechst stain indicated nuclear damage. Our study proved that V. nilgiriensis holds a strong cytotoxic effect on breast cancer cells alone and in combination with laser irradiation by upregulating the expression of apoptotic proteins such as caspase 3, p53 and Bax.

CONCLUSION

The results from this study showed that the bark ethyl acetate of V. nilgiriensis and in combination with laser is effective in preventing breast cancer cell proliferation in vitro. Further work is warranted to isolate the bioactive compounds from V. nilgiriensis bark extract and study the effect of compounds in the cell death induction. Due to the cytotoxic properties, V. nilgiriensis can be considered as a potent therapeutic agent for the treatment of cancer.

摘要

背景

癌症是指一类细胞失控繁殖的疾病。乳腺癌是女性最常见的恶性肿瘤。草药医学是大多数发展中国家重要的保健系统之一。许多研究表明，天然存在的化合物可能有助于预防和治疗各种疾病，包括癌症。一些植物提取物和分离的化合物具有光敏活性，可减少细胞增殖，而一些则具有光保护作用。

目的

尚未研究来自 V. nilgiriensis 的提取物和生物活性化合物的生物学特性和药用用途。本研究旨在评估 V. nilgiriensis 树皮提取物与 680nm 激光辐照联合对 MCF-7 乳腺癌细胞的细胞毒性作用。

方法

用倒置显微镜、ATP 和 LDH 测定法分别分析 V. nilgiriensis 树皮提取物处理后细胞形态、增殖和细胞毒性。使用波长为 680nm 和 15 J/cm2 通量的二极管激光器进行激光照射。使用 ELISA 研究凋亡蛋白的活性，用 Hoechst 染色研究核损伤。

结果

V. nilgiriensis 提取物与 680nm 激光照射联合使用可增加 MCF-7 细胞的细胞毒性并降低其增殖。Hoechst 染色结果表明核损伤。我们的研究证明，V. nilgiriensis 单独和与激光联合使用对乳腺癌细胞具有很强的细胞毒性作用，通过上调凋亡蛋白如 caspase 3、p53 和 Bax 的表达来实现。

结论

本研究结果表明，V. nilgiriensis 的乙酸乙酯树皮提取物单独使用以及与激光联合使用可有效预防体外乳腺癌细胞增殖。需要进一步工作来从 V. nilgiriensis 树皮提取物中分离生物活性化合物，并研究化合物在诱导细胞死亡中的作用。由于具有细胞毒性，V. nilgiriensis 可被视为治疗癌症的有效治疗剂。