Pattanakuhar Sintip, Kaewchur Tawika, Saiyasit Napatsorn, Chattipakorn Nipon, Chattipakorn Siriporn C
Department of Rehabilitation Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Spinal Cord. 2022 Dec;60(12):1115-1122. doi: 10.1038/s41393-022-00832-8. Epub 2022 Jul 14.
A cross-sectional study.
To investigate the correlations between gut microbiota and metabolic parameters in people with different levels of chronic spinal cord injury (SCI).
An SCI-specialized rehabilitation facility in a university hospital.
Forty-three participants with chronic SCI were recruited. Blood samples of each participant were collected for analysis of metabolic parameters. Feces were collected after the bowel opening method the patient routinely uses to evaluate fecal bacterial microbiota using quantitative RT-PCR. Body composition was examined using dual-energy x-ray absorptiometry (DEXA). Data were analyzed to evaluate the correlations between gut microbiota and other parameters.
Of the 43 participants, 31 people (72.1%) were paraplegic and 12 people (27.9%) tetraplegic. Thirty-two people (74.4%) were diagnosed with obesity using the percentage of body fat (% body fat) criteria. The mean (SD) ratio of Firmicutes:Bacteroides (F/B), which represents the degree of gut dysbiosis, was 18.3 (2.45). Using stepwise multivariable linear regression analysis, both having tetraplegia and being diagnosed with obesity from % body fat evaluated by DEXA were independent positively-correlating factors of F/B (p < 0.001 and p = 0.001, respectively), indicating more severe gut dysbiosis in people with tetraplegia than paraplegia.
In people with chronic SCI, having tetraplegia and being diagnosed with obesity from % body fat evaluated by DEXA are independent positive-correlating factors of gut dysbiosis. These results indicate a significant association between gut microbiota and the characteristics of SCI as well as metabolic parameters.
横断面研究。
探讨不同慢性脊髓损伤（SCI）程度患者肠道微生物群与代谢参数之间的相关性。
大学医院的一家SCI专科康复机构。
招募43例慢性SCI患者。采集每位参与者的血样用于分析代谢参数。采用患者常规排便后的粪便收集方法，通过定量逆转录聚合酶链反应（qRT-PCR）评估粪便细菌微生物群。使用双能X线吸收法（DEXA）检测身体成分。分析数据以评估肠道微生物群与其他参数之间的相关性。
43例参与者中，31人（72.1%）为截瘫，12人（27.9%）为四肢瘫。根据体脂百分比（%体脂）标准，32人（74.4%）被诊断为肥胖。代表肠道生态失调程度的厚壁菌门与拟杆菌门的平均（标准差）比值（F/B）为18.3（2.45）。采用逐步多变量线性回归分析，四肢瘫和根据DEXA评估的%体脂诊断为肥胖均为F/B的独立正相关因素（分别为p < 0.001和p = 0.001），表明四肢瘫患者的肠道生态失调比截瘫患者更严重。
在慢性SCI患者中，四肢瘫和根据DEXA评估的%体脂诊断为肥胖是肠道生态失调的独立正相关因素。这些结果表明肠道微生物群与SCI特征以及代谢参数之间存在显著关联。
