School of Life Sciences, University of Westminster, 115 New Cavendish Street, W1W 6UW, London, UK.
World J Microbiol Biotechnol. 2022 Jul 15;38(9):163. doi: 10.1007/s11274-022-03339-9.
Quorum quenching (QQ), a mechanism which inhibits, interferes or inactivates quorum sensing, has been investigated for control of biofilms instigated by quorum sensing process. Application of quorum quenchers (QQs) provides the possibility to investigate how different phenotypes of Pseudomonas aeruginosa (non-mucoid, mucoid, and heavily mucoid strains) modulate their gene expression to form biofilms, their quorum sensing (QS) mediated biofilm to be formed, and their virulence expressed. The mRNA expression of the AHL-mediated QS circuit and AHL-mediated virulence factors in P. aeruginosa was investigated in presence of QQs. qPCR analysis showed that farnesol and tyrosol actively reduce the expression of the synthase protein, LasI and RhlI, and prevent production of 3OC12-HSL and C4-HSL, respectively. Also, the use of farnesol and tyrosol significantly moderated gene expression for exo-proteins toxA, aprA, LasB, as well as rhlAB, which are responsible for rhamnolipid production. Our findings were promising, identifying several suppressive regulatory effects of furanone and Candida albicans QS signal molecules, tyrosol, and farnesol on the AHL-mediated P. aeruginosa QS network and related virulence factors.
群体感应淬灭（Quorum quenching，QQ）是一种抑制、干扰或失活群体感应的机制，已被用于控制由群体感应过程引发的生物膜。使用群体感应淬灭剂（Quorum quenchers，QQs）可以研究铜绿假单胞菌（非黏液型、黏液型和重度黏液型菌株）如何调节其基因表达形成生物膜、其群体感应（QS）介导的生物膜形成以及表达其毒力。研究了 QQs 存在时铜绿假单胞菌中 AHL 介导的 QS 回路和 AHL 介导的毒力因子的 mRNA 表达。qPCR 分析表明，法呢醇和酪醇可积极降低合酶蛋白 LasI 和 RhlI 的表达，并分别阻止 3OC12-HSL 和 C4-HSL 的产生。此外，法呢醇和酪醇的使用显著调节了 exo-蛋白 toxA、aprA、LasB 以及 rhlAB 的基因表达，这些蛋白负责鼠李糖脂的产生。我们的研究结果很有希望，确定了呋喃酮和白色念珠菌群体感应信号分子、酪醇和法呢醇对 AHL 介导的铜绿假单胞菌 QS 网络及相关毒力因子的几种抑制调节作用。
