RIKEN Center for Biosystems Dynamics Research, Suita, Osaka, Japan.
Methods Mol Biol. 2022;2525:47-59. doi: 10.1007/978-1-0716-2473-9_5.
By virtue of its high sensitivity, bioluminescence imaging (BLI) is an important tool for biosensing and bioimaging in life sciences. Compared to fluorescence imaging (FLI), BLI has a superior advantage that the background signals resulting from autofluorescence are almost zero due to the unnecessity of external excitation. In addition, BLI can permit a long-term observation of living cells because BL results in very low photocytotoxicity toward the host cells. Although BLI has such superior properties over FLI, the available wavelengths in BLI are mostly limited to the visible region. Here we present bioluminescence resonance energy transfer (BRET)-based visible and near-infrared dual-color molecular imaging using a quantum dot (QD) and luciferase-protein conjugate.
由于其高灵敏度，生物发光成像（BLI）是生命科学中生物传感和生物成像的重要工具。与荧光成像（FLI）相比，BLI 具有一个优越的优势，由于不需要外部激发，自发荧光产生的背景信号几乎为零。此外，BLI 可以允许对活细胞进行长期观察，因为 BL 对宿主细胞的光细胞毒性非常低。尽管 BLI 具有优于 FLI 的这些优越性质，但 BLI 中可用的波长大多限于可见区域。在这里，我们使用量子点（QD）和荧光酶-蛋白缀合物展示了基于生物发光共振能量转移（BRET）的可见和近红外双色分子成像。
