Lagadec P, Jeannin J F, Reisser D, Pelletier H, Olsson O
Invasion Metastasis. 1987;7(2):83-95.
Peritoneal carcinomatosis, a common spreading of human colon carcinoma, can be obtained by intraperitoneal injection of colon tumor cells in rats. When BDIX rats are injected with 10(6) syngeneic tumor cells, isolated and cloned from a chemically induced colon carcinoma, they die within 2-3 months with solid peritoneal tumors and hemorrhagic ascites. Repeated intraperitoneal injections of 20 micrograms endotoxins (Escherichia coli W0128:B12) from day 3 after tumor cell challenge inhibited tumor growth. This effect was long-lasting since 7 out of 10 treated rats were still alive and tumor-free 6 months after tumor cell challenge. When the endotoxins were administered from day 15 after the tumor cell challenge, in rats with established tumors visible with the naked eye, the survival times were significantly increased, and 6 out of 30 treated rats were still alive and tumor-free 6 months after tumor cell challenge. The optimum effect was obtained with 5 repeated injections. The different frequencies of injection tested, i.e. 1, 3 or 5 days apart were equally effective. Endotoxins were ineffective when administered intravenously. No side effect was observed.
腹膜癌病是人类结肠癌常见的扩散形式，可通过向大鼠腹腔内注射结肠肿瘤细胞来诱导产生。当给BDIX大鼠注射从化学诱导的结肠癌中分离并克隆出的10(6)个同基因肿瘤细胞时，它们会在2至3个月内死亡，伴有实性腹膜肿瘤和血性腹水。在肿瘤细胞接种后第3天开始，反复腹腔注射20微克内毒素（大肠杆菌W0128:B12）可抑制肿瘤生长。这种效果持久，因为在肿瘤细胞接种6个月后，10只接受治疗的大鼠中有7只仍然存活且无肿瘤。当在肿瘤细胞接种后第15天给肉眼可见已形成肿瘤的大鼠施用内毒素时，存活时间显著延长，在肿瘤细胞接种6个月后，30只接受治疗的大鼠中有6只仍然存活且无肿瘤。重复注射5次可获得最佳效果。所测试的不同注射频率，即间隔1、3或5天注射，效果相同。静脉注射内毒素无效。未观察到副作用。
