Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Locked Box 36, Pengkalan Chepa, 16100 Kota Bharu, Kelantan, Malaysia.
Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan.
Trop Biomed. 2022 Jun 1;39(2):197-202. doi: 10.47665/tb.39.2.010.
Antimicrobial resistant Escherichia coli have become an ever increasing problem in human, and animal health and production. The imprudent use of antibiotics and poor hygienic practices especially in poultry industries have been contributing to the emergence and spread of E. coli species resistant to broad spectrum antibiotics including Colistin. This study was conducted to detect colistin - resistance and antibiotic sensitivity patterns in E. coli isolated from broiler chickens in Kelantan. A total of 320 cloacal swabs were collected from apparently healthy broiler chickens in different districts of Kelantan and were analysed using routine microbiological methods, Kirby-Bauer method for antimicrobial susceptibility test and PCR amplification of species-specific and colistin - resistance encoding genes. Out of the 320 samples, 91 isolates were confirmed as E. coli and 21/91 (23.08%) were positive for colistin - resistant encoding gene, mcr-1. Most of the isolates were resistant to tetracycline (95.24%), chloramphenicol (85.71%), and sulphamethoxazole/ trimethoprim (85.71%). However, the isolates were less resistant towards piperacillin/ tazobactam (4.76%) and meropenem (9.52%). The findings from this study reveal the emerging threats of colistin - resistant in local food animal production, particularly in poultry production industry. However, more comprehensive, and large-scale studies focusing on more resistance patterns using determination of minimum inhibitory concentration (MIC), virulence and resistance characteristics and molecular epidemiology of colistin - resistant E. coli are recommended for better understanding of the epidemiology and to implement the appropriate control and prevention strategies.
耐抗生素的大肠杆菌已成为人类和动物健康与生产中日益严重的问题。抗生素的滥用和卫生条件差，尤其是在禽类行业，导致了对广谱抗生素（包括黏菌素）具有耐药性的大肠杆菌的出现和传播。本研究旨在检测来自吉兰丹州肉鸡的大肠杆菌中黏菌素耐药性和抗生素敏感性模式。从吉兰丹州不同地区的健康肉鸡中采集了 320 份直肠拭子，并使用常规微生物学方法、 Kirby-Bauer 法进行抗生素敏感性测试以及种特异性和黏菌素耐药性编码基因的 PCR 扩增进行分析。在 320 个样本中，有 91 个分离株被确认为大肠杆菌，其中 21/91（23.08%）对黏菌素耐药性编码基因 mcr-1 呈阳性。大多数分离株对四环素（95.24%）、氯霉素（85.71%）和磺胺甲恶唑/甲氧苄啶（85.71%）耐药。然而，这些分离株对哌拉西林/他唑巴坦（4.76%）和美罗培南（9.52%）的耐药性较低。本研究结果表明，当地食品动物生产，特别是禽类生产行业中，黏菌素耐药性的出现带来了新的威胁。然而，建议进行更全面、更大规模的研究，重点关注更多的耐药模式，包括使用最小抑菌浓度（MIC）测定、毒力和耐药特征以及黏菌素耐药大肠杆菌的分子流行病学，以便更好地了解其流行病学，并实施适当的控制和预防策略。
