Department of Microbiology and Veterinary Public Health, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh.
School of Agricultural, Environmental and Veterinary Sciences, Faculty of Sciences and Health, Charles Sturt University, Wagga Wagga, New South Wales, Australia.
Microb Drug Resist. 2023 Nov;29(11):523-532. doi: 10.1089/mdr.2023.0026. Epub 2023 Sep 12.
The emergence of colistin resistance in is a global public health concern. Contaminated food can accelerate the spread of colistin-resistant to humans. This study aimed to detect and characterize colistin-resistant from broiler meat in Bangladesh. We analyzed 136 pooled broiler meat samples from 240 carcasses collected from 40 live bird markets in urban and rural areas and 8 metropolitan supermarkets. The mean count of in broiler meat samples collected from rural retail shops, metropolitan supermarkets, and urban retail shops was 5.3 ± 1.1, 4.1 ± 1.4, and 3.9 ± 0.8 log colony-forming unit per gram, respectively. Colistin-resistant (minimum inhibitory concentration >2 mg/L) was found in 78% (95% confidence interval 70.2-84.1%) of the samples. All colistin-resistant isolates harbored the - gene, while the rest of the genes (- to -) were not detected. Most colistin-resistant isolates (98%) showed coresistance to tetracycline, sulfamethoxazole/trimethoprim followed by ciprofloxacin (95%). Alarmingly, all of the colistin-resistant isolates were found to be multidrug resistant. Phylogenetic analysis showed close similarities of the - gene sequences of this study with many strains of Enterobacterales isolated from humans, animals, and the environment. This study detected colistin-resistant contamination in broiler meat, which can pose a serious public health threat.
多粘菌素耐药性的出现是一个全球公共卫生关注的问题。受污染的食物会加速多粘菌素耐药性肠杆菌向人类的传播。本研究旨在检测和分析孟加拉国肉鸡肉中携带的多粘菌素耐药性肠杆菌。我们分析了 136 份来自 40 个城乡活禽市场和 8 个大都市超市的 240 只鸡只的肉鸡肉样本。从农村零售店、大都市超市和城市零售店采集的肉鸡肉样本中 的平均数量分别为 5.3±1.1、4.1±1.4 和 3.9±0.8 log 菌落形成单位/克。在 78%（95%置信区间为 70.2-84.1%）的样本中发现了多粘菌素耐药性肠杆菌（最小抑菌浓度>2 mg/L）。所有多粘菌素耐药性分离株均携带 - 基因，而其余的 基因（-至-）未被检测到。大多数多粘菌素耐药性肠杆菌分离株（98%）对四环素、磺胺甲恶唑/甲氧苄啶表现出核心耐药性，其次是环丙沙星（95%）。令人震惊的是，所有的多粘菌素耐药性分离株都被发现是多药耐药的。系统发育分析显示，本研究中 - 基因序列与从人类、动物和环境中分离的许多肠杆菌属菌株非常相似。本研究在肉鸡肉中检测到多粘菌素耐药性肠杆菌的污染，这可能对公众健康构成严重威胁。
