Clancy M J
Histochem J. 1987 Jan;19(1):27-34. doi: 10.1007/BF01675290.
Buffer solutions and incubating media for enzyme histochemistry are discussed in terms of pH, ionic strength and buffering capacity. A specially written program is presented. This program enables (i) buffers and media of known pH and ionic strength to be formulated; (ii) the ionic strength of a buffer solution of known molarity, and (iii) the thermodynamic acid dissociation constant of a buffer substance, to be calculated.
从pH值、离子强度和缓冲容量方面讨论了用于酶组织化学的缓冲溶液和孵育介质。给出了一个专门编写的程序。该程序能够：（i）配制已知pH值和离子强度的缓冲液和介质；（ii）计算已知摩尔浓度的缓冲溶液的离子强度；以及（iii）计算缓冲物质的热力学酸解离常数。
