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微秒分辨智能手机时间门控荧光光谱学。

Microsecond-resolved smartphone time-gated luminescence spectroscopy.

出版信息

Opt Lett. 2022 Jul 15;47(14):3427-3430. doi: 10.1364/OL.467458.

DOI:10.1364/OL.467458
PMID:35838696
Abstract

Time-gated luminescence spectra are usually measured by laboratory instruments equipped with high-speed excitation sources and spectrometers, which are always bulky and expensive. To reduce the reliance on expensive laboratory instruments, we demonstrate the first, to the best of our knowledge, use of a smartphone for the detection of time-gated luminescence spectra. A mechanical chopper is used as the detection shutter and an optical switch is placed at the edge of the wheel to convert the chopping signal into a transistor-transistor logic (TTL) signal which is used to control the excitation source and achieve synchronization. The time-gated luminescence spectra at different delay times of Eu(TTA) powder and the solutions of Eu-tetracycline complex are successfully detected with a temporal resolution of tens of microseconds by the proposed approach. We believe our approach offers a route toward portable instruments for the measurement of luminescence spectra and lifetimes.

摘要

时间门控荧光光谱通常由配备高速激发源和光谱仪的实验室仪器测量,这些仪器通常体积庞大且价格昂贵。为了减少对昂贵实验室仪器的依赖,我们首次展示了,据我们所知,使用智能手机来检测时间门控荧光光谱。一个机械斩波器被用作检测快门,一个光开关被放置在轮子的边缘,将斩波信号转换成晶体管-晶体管逻辑(TTL)信号,用于控制激发源并实现同步。通过所提出的方法,成功地检测了 Eu(TTA)粉末和 Eu-四环素配合物溶液在不同延迟时间的时间门控荧光光谱,时间分辨率为几十微秒。我们相信,我们的方法为便携式荧光光谱和寿命测量仪器提供了一种途径。

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引用本文的文献

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Development of Time-Resolved Luminescence Measurement Instruments for Biosensing and Bioimaging - An Overview.用于生物传感和生物成像的时间分辨发光测量仪器的发展——综述
Measurement (Lond). 2025 Jun 15;250. doi: 10.1016/j.measurement.2025.117201. Epub 2025 Mar 6.
2
Low-rate smartphone videoscopy for microsecond luminescence lifetime imaging with machine learning.用于机器学习的微秒级发光寿命成像的低帧率智能手机视频显微镜技术
PNAS Nexus. 2023 Sep 27;2(10):pgad313. doi: 10.1093/pnasnexus/pgad313. eCollection 2023 Oct.