Wuhan National Lab for Optoelectronics and Key Lab of Materials Chemistry for Energy Conversion and Storage of Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, Hubei, China.
Hubei Key Lab of Biomass Fibers and Eco-dyeing & Finishing, Department of Chemistry and Chemical Engineering, Wuhan Textile University, Wuhan, 430073, Hubei, China.
Anal Bioanal Chem. 2021 May;413(12):3291-3297. doi: 10.1007/s00216-021-03266-y. Epub 2021 Mar 26.
Luminescence lifetime imaging plays an important role in distinguishing the luminescence decay rates in time-resolved luminescence imaging. However, traditional imaging instruments used for detecting lifetimes within milliseconds would be time-consuming when imaging ultra-long luminescence lifetimes over subseconds. Herein, we present an accessible and simple optical system for detecting lifetimes of persistent luminescence. A smartphone integrated with a UV LED, a dichroic mirror, and a lens was used for recording the persistent luminescence. With only a few seconds of data acquisition, a luminescence lifetime image could be processed from the video by exponential fitting of the gray level of each pixel to the delay time. Since this approach only requires single excitation, no synchronous control is needed, greatly simplifying the apparatus and saving the cost. The apparatus was successfully used for ultra-long luminescence lifetime imaging of mouse tissue dyed with a persistent luminescence molecule. This miniaturized apparatus exhibits huge potentiality in time-resolved luminescence imaging for luminescence study and biological detection.
荧光寿命成像是区分时间分辨荧光成像中荧光衰减率的重要手段。然而，传统的用于检测毫秒范围内寿命的成像仪器在对亚秒级的超长荧光寿命进行成像时会非常耗时。在此，我们提出了一种用于检测持续发光寿命的简单易用的光学系统。一个集成有紫外发光二极管、二向色镜和透镜的智能手机被用于记录持续发光。通过对每个像素的灰度值与延迟时间进行指数拟合，仅需几秒钟的数据采集，即可从视频中处理出荧光寿命图像。由于这种方法仅需要单次激发，因此不需要同步控制，大大简化了仪器并降低了成本。该仪器成功地用于对用持续发光分子染色的小鼠组织进行超长荧光寿命成像。这种小型化的仪器在时间分辨荧光成像方面具有很大的潜力，可用于发光研究和生物检测。
