Department of Biophysics, School of Basic Medical Sciences, Health Science Centre, Xi'an Jiaotong University, Shaanxi 710061, P. R. China.
Active Soft Matter Group, CAS Songshan Lake Materials Laboratory, Dongguan 523808, China.
Langmuir. 2022 Jul 26;38(29):8733-8747. doi: 10.1021/acs.langmuir.2c00711. Epub 2022 Jul 15.
This feature article introduces the design of self-assembling peptides that serve as the basic building blocks for the construction of extracellular matrix (ECM)-like structure in the vicinity of the plasma membrane. By covalently conjugating a bioactive motif, such as membrane protein binding ligand or enzymatic responsive building block, with a self-assembling motif, especially the aromatic peptide, a self-assembling peptide that retains bioactivity is obtained. Instructed by the target membrane protein or enzyme, the bioactive peptides self-assemble into ECM-like structure exerting various stimuli to regulate the cellular response via intracellular signaling, especially mechanotransduction. By briefly summarizing the properties and applications (e.g., wound healing, controlling cell motility and cell fate) of these peptides, we intend to illustrate the basic requirements and promises of the peptide assembly as a true bottom-up approach in the construction of artificial ECM.
这篇专题文章介绍了自组装肽的设计，这些肽可作为构建细胞膜附近细胞外基质（ECM）样结构的基本构建块。通过将生物活性基序（例如膜蛋白结合配体或酶响应构建基序）与自组装基序（特别是芳香族肽）共价连接，获得保留生物活性的自组装肽。在目标膜蛋白或酶的指导下，生物活性肽自组装成 ECM 样结构，通过细胞内信号传导施加各种刺激来调节细胞反应，特别是机械转导。通过简要总结这些肽的性质和应用（例如，伤口愈合、控制细胞迁移和细胞命运），我们旨在说明肽组装作为人工 ECM 构建的真正自下而上方法的基本要求和前景。
