Redondo-Gómez Carlos, Padilla-Lopategui Soraya, Azevedo Helena S, Mata Alvaro
School of Engineering & Materials Science, Queen Mary University of London, London E1 4NS, U.K.
Institute of Bioengineering, Queen Mary University of London, London E1 4NS, U.K.
ACS Biomater Sci Eng. 2020 Sep 14;6(9):4870-4880. doi: 10.1021/acsbiomaterials.0c00549. Epub 2020 Aug 17.
A key feature in biomaterial design is the incorporation of bioactive signals into artificial constructs to stimulate tissue regeneration. Most currently used hydrogel cell culture systems depend on the covalent attachment of extracellular matrix (ECM)-derived peptides to either macromolecular units or smaller self-assembling building blocks, thereby restricting biosignal presentation and adaptability. However, new ways to rationally incorporate adhesion epitopes through noncovalent interactions would offer opportunities to better recreate the dynamic and reversible nature of the native ECM. Here, we report on a noncovalent epitope presentation approach mediated by host-guest interactions. Using peptide amphiphile hydrogels, we demonstrate that the adamantane/β-cyclodextrin pair can be used to anchor RGDS cell adhesion signals onto self-assembled hydrogels host-guest interactions. We evaluate hydrogel morphological and rheological properties as well as fibroblast attachment, organization, and spreading when cultured atop these scaffolds. This host-guest-mediated epitope display might lead to new self-assembling hydrogels for improved cell culture applications in fields such as tissue engineering and regenerative medicine.
生物材料设计的一个关键特征是将生物活性信号整合到人工构建体中以刺激组织再生。目前大多数使用的水凝胶细胞培养系统依赖于将细胞外基质（ECM）衍生的肽共价连接到大分子单元或较小的自组装构建块上，从而限制了生物信号的呈现和适应性。然而，通过非共价相互作用合理整合粘附表位的新方法将为更好地重现天然ECM的动态和可逆性质提供机会。在此，我们报道一种由主客体相互作用介导的非共价表位呈现方法。使用肽两亲性水凝胶，我们证明金刚烷/β-环糊精对可用于通过主客体相互作用将RGDS细胞粘附信号锚定到自组装水凝胶上。我们评估了水凝胶的形态和流变学性质，以及在这些支架上培养时成纤维细胞的附着、组织和铺展情况。这种主客体介导的表位展示可能会产生新的自组装水凝胶，用于改善组织工程和再生医学等领域的细胞培养应用。
