State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
School of Biotechnology and Food Engineering, Changshu Institute of Technology, Changshu, Jiangsu 215500, PR China.
Food Chem. 2022 Dec 1;396:133660. doi: 10.1016/j.foodchem.2022.133660. Epub 2022 Jul 9.
Amanitin (AMA) is responsible for human fatalities after ingestion of poisonous mushrooms, thus, a rapid and accurate detection method is urgently needed. Here, gold nanoparticle-based immunosensor with monoclonal antibody against AMA was constructed for rapid detection of alpha- and beta-amanitin (α- and β-AMA) in mushroom, serum and urine samples. Under optimized conditions, the visual limits of detection (vLOD) and calculated LOD for α-AMA and β-AMA in mushroom were 10 ng/g, 20 ng/g, and 0.1 ng/g, 0.2 ng/g, respectively. Analysis of wild mushroom samples was also performed using a strip scan reader in the 10 min range. Furthermore, in mushrooms containing amatoxins results were confirmed and compared with those determined by liquid chromatography tandem mass spectrometry. Thus, this immunosensor provided a useful monitoring tool for rapid detection and screening of mushroom samples and in serum and urine from subjects who accidentally consumed AMA-containing mushrooms.
鹅膏肽（AMA）是食用毒蘑菇后导致人类死亡的罪魁祸首，因此，急需一种快速、准确的检测方法。本研究构建了一种基于金纳米颗粒的 AMA 单克隆抗体免疫传感器，用于快速检测蘑菇、血清和尿液样本中的α-和β-鹅膏肽（α-和β-AMA）。在优化条件下，该免疫传感器对蘑菇中 α-AMA 和 β-AMA 的可视检测限（vLOD）和计算检测限分别为 10 ng/g、20 ng/g 和 0.1 ng/g、0.2 ng/g。采用条带扫描阅读器在 10 分钟内对野生蘑菇样本进行了分析。此外，对含有鹅膏肽的蘑菇样本进行了检测，结果与液相色谱串联质谱法的测定结果进行了比较。因此，该免疫传感器为快速检测和筛选蘑菇样本以及意外摄入 AMA 蘑菇的个体的血清和尿液提供了一种有用的监测工具。
