Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA.
Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA.
STAR Protoc. 2022 Sep 16;3(3):101543. doi: 10.1016/j.xpro.2022.101543. Epub 2022 Jul 14.
This protocol describes an ex vivo cell culture system for simultaneously generating a mixture of CD4 T helper lineages, including T helper 17 (Th17), RORγt Treg, and conventional Treg (cTreg), in proportions representative of those found in mucosal tissues in vivo. When combined with a co-culture approach, this system allows a more rapid assessment of a candidate molecule's T cell-intrinsic and -extrinsic functions over the traditional bone marrow chimera and co-transfer approaches. For complete details on the use and execution of this protocol, please refer to Ma et al. (2022).
本方案描述了一种在体外用细胞培养系统，可在体外按体内粘膜组织中发现的比例同时产生混合的 CD4 辅助性 T 细胞谱系，包括辅助性 T 细胞 17（Th17）、RORγt Treg 和常规 Treg（cTreg）。当与共培养方法结合使用时，该系统可更快速地评估候选分子的 T 细胞内在和外在功能，而无需采用传统的骨髓嵌合体和共转移方法。如需了解该方案的详细使用和实施方法，请参考 Ma 等人（2022 年）。
