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Jiahang Deng, Xinyue Feng, Longjian Zhou, Chuantong He, Huilin Li, Juan Xia, Yuewei Ge, Yuntao Zhao, Cai Song, Lei Chen, Zhiyou Yang
College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China.
College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China.
Food Res Int. 2022 Aug;158:111576. doi: 10.1016/j.foodres.2022.111576. Epub 2022 Jun 25.
Pseudostellaria heterophylla, has historically been used as medicine food homology plant for thousand years in China. Our previous studies had indicated that daily intake of Pseudostellaria heterophylla extract enhanced cognitive memory. Herein, heterophyllin B (HET-B), a brain permeable cyclopeptide from Pseudostellaria heterophylla was determined, and the molecular mechanism underlying its memory improvement effects was investigated. Pseudostellaria heterophylla extract as well as HET-B reversed Aβ25-35-induced axonal atrophy and neuronal apoptosis in cultured cortical neurons of mice. HET-B could enhance memory retrieval, modulate splenic T helper cell, and ameliorate neuroinflammation in i.c.v. Aβ1-42 injected Alzheimer's disease (AD) mice. To explore the mechanism of action, network pharmacology was performed to predict protein targets and pathways of HET-B against AD. Five key targets were identified related to the effect of HET-B in AD intervention, and were clarified involved in axonal regeneration. We revealed for the first time that HET-B promoted memory retrieval through axonal regeneration and anti-neuroinflammation. This study provides a basis to research on HET-B as nutritional supplements for brain healthy.
三叶准噶尔参在我国作为药食同源植物已有千年历史。我们之前的研究表明，每日摄入三叶准噶尔参提取物可增强认知记忆。本文从三叶准噶尔参中鉴定出一种可穿透血脑屏障的环肽化合物异叶准噶尔参苷 B（HET-B），并研究了其改善记忆的作用机制。三叶准噶尔参提取物和 HET-B 均可逆转 Aβ25-35 诱导的培养小鼠皮质神经元轴突萎缩和神经元凋亡。HET-B 可增强记忆检索、调节脾辅助性 T 细胞，并改善 i.c.v. Aβ1-42 注射阿尔茨海默病（AD）小鼠的神经炎症。为了探讨作用机制，采用网络药理学预测 HET-B 对 AD 的蛋白靶点和通路。鉴定出与 HET-B 干预 AD 相关的 5 个关键靶点，这些靶点与轴突再生有关。我们首次揭示 HET-B 通过促进轴突再生和抗神经炎症来促进记忆检索。本研究为将 HET-B 作为大脑健康的营养补充剂的研究提供了依据。
