Wang Chongmin, Yang Jiaotong, Pan Qi, Zhu Panpan, Li Jun
Guizhou University of Traditional Chinese Medicine, Guiyang, China.
Front Nutr. 2024 Jul 5;11:1417526. doi: 10.3389/fnut.2024.1417526. eCollection 2024.
Abscisic acid (ABA) significantly regulates plant growth and development, promoting tuberous root formation in various plants. However, the molecular mechanisms of ABA in the tuberous root development of are not yet fully understood. This study utilized Illumina sequencing and assembly strategies to obtain a reference transcriptome associated with ABA treatment. Subsequently, integrated transcriptomic and proteomic analyses were used to determine gene expression profiles in tuberous roots. ABA treatment significantly increases the diameter and shortens the length of tuberous roots. Clustering analysis identified 2,256 differentially expressed genes and 679 differentially abundant proteins regulated by ABA. Gene co-expression and protein interaction networks revealed ABA positively induced 30 vital regulators. Furthermore, we identified and assigned putative functions to transcription factors (PhMYB10, PhbZIP2, PhbZIP, PhSBP) that mediate ABA signaling involved in the regulation of tuberous root development, including those related to cell wall metabolism, cell division, starch synthesis, hormone metabolism. Our findings provide valuable insights into the complex signaling networks of tuberous root development modulated by ABA. It provided potential targets for genetic manipulation to improve the yield and quality of , which could significantly impact its cultivation and medicinal value.
脱落酸（ABA）显著调节植物的生长发育，促进多种植物块根的形成。然而，ABA在[植物名称]块根发育中的分子机制尚未完全阐明。本研究利用Illumina测序和[组装策略名称]组装策略获得了与ABA处理相关的参考转录组。随后，采用转录组和蛋白质组综合分析来确定[植物名称]块根中的基因表达谱。ABA处理显著增加了块根的直径并缩短了其长度。聚类分析确定了2256个受ABA调控的差异表达基因和679个差异丰度蛋白质。基因共表达和蛋白质相互作用网络显示ABA正向诱导了30个关键调节因子。此外，我们鉴定并赋予了介导ABA信号传导参与块根发育调控的转录因子（PhMYB10、PhbZIP2、PhbZIP、PhSBP）假定功能，这些调控包括与细胞壁代谢、细胞分裂、淀粉合成、激素代谢相关的调控。我们的研究结果为ABA调节的块根发育复杂信号网络提供了有价值的见解。它为基因操作提供了潜在靶点，以提高[植物名称]的产量和品质，这可能对其种植和药用价值产生重大影响。
