SYNGR2 可作为预测生物标志物，与食管鳞状细胞癌中的免疫浸润相关。

SYNGR2 serves as a prognostic biomarker and correlates with immune infiltrates in esophageal squamous cell carcinoma.

机构信息

Department of Thoracic Surgery, Lanzhou University Second Hospital, Lanzhou University Second Clinical Medical College, Lanzhou, China.

出版信息

J Gene Med. 2022 Aug;24(8):e3441. doi: 10.1002/jgm.3441. Epub 2022 Aug 1.

DOI:10.1002/jgm.3441

PMID:35840542

Abstract

BACKGROUND

Synaptogyrin-2 (SYNGR2) plays an important role in regulating membrane traffic in non-neuronal cells. However, the role of SYNGR2 in esophageal squamous cell carcinoma (ESCC) remains unclear.

METHODS

All original data were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases and integrated via R 3.5.3. SYNGR2 expression was explored in the TCGA and GEO databases. The correlations between SYNGR2 and cancer immune characteristics were analyzed via the TIMER and TISIDB databases.

RESULTS

In general, SYNGR2 was predominantly overexpressed and had reference values in the diagnosis and prognostic estimation of ESCC. Upregulated SYNGR2 was associated with poorer overall survival, disease-specific survival and T stage in ESCC. Mechanistically, we identified hub genes that included a total of 38 SYNGR2-related genes, which were tightly associated with the protein polyubiquitination pathway in ESCC patients. SYNGR2 expression was negatively related to the infiltrating levels of T helper cells. SYNGR2 methylation was positively correlated with the expression of chemokines (CCL2 and CXCL12), chemokine receptors (CCR1 and CCR2), immunoinhibitors (CXCL12 and TNFRSF4) and immunostimulators (CSF1R and PDCD1LG2) in ESCC.

CONCLUSION

SYNGR2 may be used as a biomarker for determining prognosis and immune infiltration in ESCC.

摘要

背景

突触融合蛋白 2（SYNGR2）在非神经元细胞中调节膜转运方面发挥着重要作用。然而，SYNGR2 在食管鳞状细胞癌（ESCC）中的作用尚不清楚。

方法

所有原始数据均从癌症基因组图谱（TCGA）和基因表达综合数据库（GEO）下载，并通过 R 3.5.3 进行整合。在 TCGA 和 GEO 数据库中探索 SYNGR2 的表达。通过 TIMER 和 TISIDB 数据库分析 SYNGR2 与癌症免疫特征的相关性。

结果

总体而言，SYNGR2 在 ESCC 的诊断和预后估计中表现出高表达和参考价值。上调的 SYNGR2 与 ESCC 患者的总生存期、疾病特异性生存期和 T 分期较差相关。从机制上讲，我们确定了包含总共 38 个 SYNGR2 相关基因的枢纽基因，这些基因与 ESCC 患者的蛋白质多泛素化途径密切相关。SYNGR2 的表达与辅助性 T 细胞的浸润水平呈负相关。SYNGR2 的甲基化与趋化因子（CCL2 和 CXCL12）、趋化因子受体（CCR1 和 CCR2）、免疫抑制剂（CXCL12 和 TNFRSF4）和免疫刺激剂（CSF1R 和 PDCD1LG2）在 ESCC 中的表达呈正相关。