Graduate School of Life Sciences, Tohoku University, 6-3 Aramaki aza aoba, Aoba-ku, Sendai, Miyagi, 980-8578, Japan.
Japan Wildlife Research Center, Kotobashi 3-3-7 Sumida-ku Tokyo, Tokyo, 130-8606, Japan.
BMC Res Notes. 2022 Jul 16;15(1):255. doi: 10.1186/s13104-022-06147-4.
Mandarina is an endangered land snail genus of the oceanic Ogasawara archipelago. On Chichijima Island, the largest inhabited island in Ogasawara, this genus is almost extinct in the wild due to predation by invasive species. Although ex situ conservation programs started in 2010, genetic diversity and population structure remain unclear due to a lack of genetic markers with sufficient genetic variation. In this study, we designed polymorphic microsatellite markers of Mandarina to enable genetic analysis and to develop appropriate conservation plans.
Twenty-three polymorphic microsatellite markers were identified from the genomic DNA of wild samples of Mandarina mandarina. We assessed the genetic diversity of each marker. In 16 markers, neither linkage disequilibrium nor deviation from Hardy-Weinberg equilibrium was detected. These 16 markers were tested for multiplex PCR using low-density DNA extracted non-lethally from captive samples of M. mandarina, M. chichijimana and M. suenoae. Of the 16 markers, 15, 12 and 9 were usable for multiplex PCR, respectively. Genetic analysis using these microsatellite loci will be an important resource for the conservation of Mandarina.
Mandarina 是海洋小笠原群岛特有的濒危陆地蜗牛属。在小笠原群岛最大的有人居住的岛屿——父岛，由于入侵物种的捕食，该属在野外几近灭绝。尽管自 2010 年以来已经开始实施就地保护计划，但由于缺乏具有足够遗传变异的遗传标记，遗传多样性和种群结构仍不清楚。在这项研究中，我们设计了 Mandarina 的多态性微卫星标记，以进行遗传分析并制定适当的保护计划。
从野生 Mandarina mandarina 样本的基因组 DNA 中鉴定出 23 个多态性微卫星标记。我们评估了每个标记的遗传多样性。在 16 个标记中，均未检测到连锁不平衡或偏离 Hardy-Weinberg 平衡。使用从圈养的 M. mandarina、M. chichijimana 和 M. suenoae 样本中提取的非致死性低密度 DNA 对这 16 个标记进行了多重 PCR 测试。在这 16 个标记中，分别有 15、12 和 9 个标记可用于多重 PCR。使用这些微卫星位点进行的遗传分析将成为保护 Mandarina 的重要资源。
