COLGAIT1 是预测肾透明细胞癌预后和免疫反应的潜在生物标志物及其 ceRNA 网络的机制。
COLGALT1 is a potential biomarker for predicting prognosis and immune responses for kidney renal clear cell carcinoma and its mechanisms of ceRNA networks.
机构信息
Department of Urology, Shanghai General Hospital of Nanjing Medical University, Shanghai, 200080, China.
Department of Urology, Affiliated Hospital of Nantong University, Jiangsu Province, Nantong, 226001, China.
出版信息
Eur J Med Res. 2022 Jul 16;27(1):122. doi: 10.1186/s40001-022-00745-5.
BACKGROUND
As precision medicine gradually played an inaccessible role in cancer treatment, there was an urgent need to explore biomarkers or signatures for predicting cancer prognosis. Currently, little was known about the associations between COLGALT1 and kidney renal clear cell carcinoma (KIRC). Hence, this study was performed to reveal its roles in KIRC and to identify potential mechanisms of competing endogenous RNA (ceRNA) networks.
METHODS
R 4.1.1 software was utilized to conduct bioinformatics analyses with the data derived from online databases. Difference analysis, survival analysis, univariate/multivariate cox regression analysis and correlation analysis were carried out successively in this article. Besides, we also investigated potential effects and mechanisms of COLGALT1 in KIRC.
RESULTS
COLGALT1 expression was overexpressed in KIRC samples compared with the normal samples and it was associated with poor OS (P < 0.001). COLGALT1 was also found to be significantly related to clinicopathological characteristics such as grade, T, N, M, stage and Cox regression analysis with univariate and multivariate data suggested it might be an independent prognostic parameter in KIRC (P < 0.001). Furthermore, Seven significantly enriched pathways were identified. Interestingly, correlation analyses revealed an association between COLGALT1 and microsatellite instability (MSI), tumor mutational burden (TMB) and immunity (P < 0.001). In addition, we used TIDE and TCIA databases to predict the immune response of COLGALT1 in KIRC and it suggested low expression of COLGALT1 is more likely to benefit from immunotherapy. Besides, we identified a ceRNA network of SLC16A1-AS1/hsa-mir-502-3p/COLGALT1 for its potential mechanism. Finally, experiments in vitro indicated that COLGALT1 was significantly related to cell proliferation.
CONCLUSIONS
COLGALT1 could act as a valid immune-related prognostic indicator for KIRC and participated in a ceRNA network of SLC16A1-AS1/hsa-mir-502-3p/COLGALT1, offering one potential biomarker to investigate the mechanism and clinical therapeutic value of KIRC.
背景
随着精准医学在癌症治疗中逐渐发挥不可企及的作用，迫切需要探索预测癌症预后的生物标志物或特征。目前，关于 COLGALT1 与肾透明细胞癌（KIRC）之间的关联知之甚少。因此，本研究旨在揭示其在 KIRC 中的作用，并确定竞争内源性 RNA（ceRNA）网络的潜在机制。
方法
使用 R 4.1.1 软件对来自在线数据库的数据进行生物信息学分析。本文依次进行差异分析、生存分析、单变量/多变量 Cox 回归分析和相关性分析。此外，我们还研究了 COLGALT1 在 KIRC 中的潜在影响和机制。
结果
与正常样本相比，KIRC 样本中 COLGALT1 的表达上调，并且与 OS 不良相关（P < 0.001）。COLGALT1 还与病理特征显著相关，如分级、T、N、M、分期，单变量和多变量 Cox 回归分析表明其可能是 KIRC 的独立预后参数（P < 0.001）。此外，还确定了七个显著富集的途径。有趣的是，相关性分析显示 COLGALT1 与微卫星不稳定性（MSI）、肿瘤突变负担（TMB）和免疫之间存在关联（P < 0.001）。此外，我们使用 TIDE 和 TCIA 数据库预测 KIRC 中 COLGALT1 的免疫反应，结果表明 COLGALT1 低表达更可能受益于免疫治疗。此外，我们确定了 SLC16A1-AS1/hsa-mir-502-3p/COLGALT1 的 ceRNA 网络，以研究其潜在机制。最后，体外实验表明 COLGALT1 与细胞增殖显著相关。
结论
COLGALT1 可作为 KIRC 的有效免疫相关预后指标，并参与 SLC16A1-AS1/hsa-mir-502-3p/COLGALT1 的 ceRNA 网络，为研究 KIRC 的机制和临床治疗价值提供了一个潜在的生物标志物。
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