School of BioSciences, University of Melbourne, Parkville, Vic., 3010, Australia.
Max Planck Institute of Molecular Plant Physiology, 14476, Potsdam, Germany.
New Phytol. 2022 Nov;236(3):1027-1041. doi: 10.1111/nph.18380. Epub 2022 Aug 5.
Sugars are essential metabolites for energy and anabolism that can also act as signals to regulate plant physiology and development. Experimental tools to disrupt major sugar signalling pathways are limited. We performed a chemical screen for modifiers of activation of circadian gene expression by sugars to discover pharmacological tools to investigate and manipulate plant sugar signalling. Using a library of commercially available bioactive compounds, we identified 75 confident hits that modified the response of a circadian luciferase reporter to sucrose in dark-adapted Arabidopsis thaliana seedlings. We validated the transcriptional effect on a subset of the hits and measured their effects on a range of sugar-dependent phenotypes for 13 of these chemicals. Chemicals were identified that appear to influence known and unknown sugar signalling pathways. Pentamidine isethionate was identified as a modifier of a sugar-activated Ca signal that acts as a calmodulin inhibitor downstream of superoxide in a metabolic signalling pathway affecting circadian rhythms, primary metabolism and plant growth. Our data provide a resource of new experimental tools to manipulate plant sugar signalling and identify novel components of these pathways.
糖是能量和合成代谢的必需代谢物，也可以作为信号来调节植物的生理和发育。破坏主要糖信号通路的实验工具是有限的。我们进行了化学筛选，以寻找能够激活生物钟基因表达的调节剂，从而发现药理学工具来研究和操纵植物糖信号。我们使用了商业上可用的生物活性化合物文库，鉴定出 75 种有信心的化合物能够改变黑暗适应的拟南芥幼苗中蔗糖对生物钟荧光素酶报告基因表达的响应。我们验证了对其中一部分化合物的转录效应，并测量了它们对这 13 种化合物中 13 种糖依赖性表型的影响。鉴定出的化合物似乎影响了已知和未知的糖信号通路。戊二脒乙磺酸盐被鉴定为一种能够调节糖激活的 Ca 信号的调节剂，它作为超氧化物下游的钙调蛋白抑制剂，在影响生物钟节律、初级代谢和植物生长的代谢信号通路中发挥作用。我们的数据提供了一种新的实验工具资源，用于操纵植物糖信号，并确定这些通路的新成分。
