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优化的从小鼠多能干细胞中获得生殖系干细胞样细胞的方案。

Optimized protocol to derive germline stem-cell-like cells from mouse pluripotent stem cells.

机构信息

Institute for the Advanced Study of Human Biology (WPI-ASHBi), Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan; Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.

Institute for the Advanced Study of Human Biology (WPI-ASHBi), Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan; Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.

出版信息

STAR Protoc. 2022 Sep 16;3(3):101544. doi: 10.1016/j.xpro.2022.101544. Epub 2022 Jul 16.

DOI:10.1016/j.xpro.2022.101544
PMID:35842863
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9294266/
Abstract

Male germ-cell development comprises primordial germ-cell (PGC) development, spermatogonium differentiation, and ensuing spermatogenesis. We present a step-by-step protocol for differentiation of mouse pluripotent stem cells (PSCs) into germline stem-cell-like cells (GSCLCs) via PGC-like cell and spermatogonium-like cell intermediates. The differentiation protocol has higher fidelity than our previous protocol. Upon transplantation into testes in vivo or culture for testis transplants, GSCLCs robustly contribute to spermatogenesis, providing a paradigm for PSC-based reconstitution of mammalian male germ-cell development. For complete details on the use and execution of this protocol, please refer to Ishikura et al. (2021).

摘要

雄性生殖细胞的发育包括原始生殖细胞（PGC）的发育、精原细胞的分化以及随后的精子发生。我们提出了一个逐步的方案，通过 PGC 样细胞和精原细胞样细胞中间产物，将小鼠多能干细胞（PSCs）分化为生殖干细胞样细胞（GSCLCs）。该分化方案比我们之前的方案具有更高的保真度。在体内移植到睾丸或睾丸移植培养中，GSCLCs 可强有力地促进精子发生，为基于 PSC 的哺乳动物雄性生殖细胞发育重建提供了范例。如需详细了解本方案的使用和执行情况，请参阅 Ishikura 等人（2021 年）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/71e508c6ddf2/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/8bbbb245920c/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/0b701a986797/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/5ca2fa9ff680/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/a963dfb0463c/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/e1d2af8ffde4/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/17a900683a4d/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/c212f06013b9/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/5052dc1d7d40/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/cb28e4a9867a/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/71e508c6ddf2/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/8bbbb245920c/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/0b701a986797/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/5ca2fa9ff680/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/a963dfb0463c/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/e1d2af8ffde4/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/17a900683a4d/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/c212f06013b9/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/5052dc1d7d40/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/cb28e4a9867a/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/172c/9294266/71e508c6ddf2/gr9.jpg

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