Department of Respiratory Diseases, Univ Montpellier, CHU Montpellier, Montpellier, France.
Clinical Proteomics Platform, LBPC, IRMB, CHU Montpellier, Montpellier University, Montpellier, France.
Biochem Biophys Res Commun. 2022 Sep 24;622:64-71. doi: 10.1016/j.bbrc.2022.07.025. Epub 2022 Jul 11.
Mucus is known to play a pathogenic role in muco-obstructive lung diseases, but little is known about the determinants of mucus rheology. The purpose of this study is to determine which sputum components influence sputum rheology in patients with muco-obstructive lung diseases.
We performed a cross sectional prospective cohort study. Spontaneous sputum was collected from consecutive patients with muco-obstructive lung diseases. Sputum rheology was assessed using the Rheomuco® rheometer (Rheonova, Grenoble); the elastic modulus G', viscous modulus G″, and the critical stress threshold σc were recorded. Key quantitative and qualitative biological sputum components were determined by cytology, nucleic acid amplification tests and mass spectrometry.
48 patients were included from January to August 2019. Among them, 10 had asthma, 14 COPD and 24 non-CF bronchiectasis (NCFB). The critical stress threshold σc predicted a sputum eosinophilia superior to 1.25% with 89.19% accuracy (AUC = 0.8762). G' and G″ are positively correlated with MUC5AC protein concentration ((rho = 0.361; P = .013) and (rho = 0.335; P = .021), respectively). σc was positively correlated with sputum eosinophilia (rho = 0.394; P = .012), MUC5B (rho = 0.552; P < .001) and total protein (rho = 0.490; P < .001) concentrations. G' and G″ were significantly higher in asthma patients (G' = 14.49[7.18-25.26]Pa, G'' = 3.0[2.16-5.38]Pa) compared to COPD (G' = 5.01[2.94-6.48]Pa, P = .010; G'' = 1.45[1.16-1.94]Pa, P = .006) and to NCFB (G' = 4.99[1.49-10.49]Pa, P = .003; G'' = 1.46[0.71-2.47]Pa, P = .002).
In muco-obstructive lung diseases, rheology predicts sputum eosinophilia and is correlated with mucin concentrations, regardless of the underlying disease.
(registrar, website, and registration number), where applicable NCT04081740.
黏液在黏液阻塞性肺部疾病中起着致病作用，但人们对黏液流变学的决定因素知之甚少。本研究的目的是确定哪些痰液成分会影响黏液阻塞性肺部疾病患者的痰液流变学。
我们进行了一项横断面前瞻性队列研究。连续收集黏液阻塞性肺部疾病患者的自发性痰液。使用 Rheomuco®流变仪（Rheonova，格勒诺布尔）评估痰液流变学；记录弹性模量 G'、粘性模量 G″和临界应力阈值σc。通过细胞学、核酸扩增试验和质谱法确定关键的定量和定性生物痰液成分。
2019 年 1 月至 8 月期间纳入 48 例患者，其中 10 例为哮喘，14 例为 COPD，24 例为非 CF 支气管扩张症（NCFB）。临界应力阈值σc可预测痰嗜酸性粒细胞超过 1.25%，准确率为 89.19%（AUC=0.8762）。G'和 G″与 MUC5AC 蛋白浓度呈正相关（rho=0.361；P=0.013）和（rho=0.335；P=0.021）。σc与痰嗜酸性粒细胞（rho=0.394；P=0.012）、MUC5B（rho=0.552；P<0.001）和总蛋白（rho=0.490；P<0.001）浓度呈正相关。与 COPD（G'=5.01[2.94-6.48]Pa，P=0.010；G''=1.45[1.16-1.94]Pa，P=0.006）和 NCFB（G'=4.99[1.49-10.49]Pa，P=0.003；G''=1.46[0.71-2.47]Pa，P=0.002）相比，哮喘患者的 G'和 G″显著更高（G'=14.49[7.18-25.26]Pa，G''=3.0[2.16-5.38]Pa）。
在黏液阻塞性肺部疾病中，流变学可预测痰嗜酸性粒细胞，并与黏蛋白浓度相关，无论潜在疾病如何。
（注册机构、网站和注册号），适用时 NCT04081740。
