Arbatsky Nikolay P, Shashkov Alexander S, Shneider Mikhail M, Popova Anastasiya V, Kasimova Anastasiya A, Miroshnikov Konstantin A, Knirel Yuriy A, Hall Ruth M, Kenyon Johanna J
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Int J Biol Macromol. 2022 Sep 30;217:515-521. doi: 10.1016/j.ijbiomac.2022.07.085. Epub 2022 Jul 14.
Acinetobacter baumannii isolate LUH5552 carries the KL89 capsule biosynthesis gene cluster. Capsular polysaccharide (CPS) isolated from LUH5552 was analyzed by sugar analysis, Smith degradation, and one- and two-dimensional H and C NMR spectroscopy. The K89 CPS structure has not been seen before in A. baumannii CPS structures resolved to date and includes a 3-acetamido-3,6-dideoxy-d-galactose (d-Fucp3NAc) residue which is rare amongst A. baumannii CPS. The K89 CPS has a →3)-α-d-GalpNAc-(1→3)-β-d-GlcpNAc-(1→ main chain with a β-d-Glcp-(1→2)-β-d-Fucp3NAc-(1→6)-d-Glcp side branch that is α-(1→4) linked to d-GalpNAc. The roles of the Wzy polymerase and the four glycosyltransferases encoded by the KL89 gene cluster in the biosynthesis of the K89 CPS were assigned. Two glycosyltransferases, Gtr121 and Gtr122, link the d-Fucp3NAc to its neighboring sugars.
鲍曼不动杆菌分离株LUH5552携带KL89荚膜生物合成基因簇。通过糖分析、史密斯降解以及一维和二维氢谱与碳谱核磁共振波谱对从LUH5552分离出的荚膜多糖（CPS）进行了分析。K89 CPS结构在迄今为止解析的鲍曼不动杆菌CPS结构中从未见过，并且包含一个3-乙酰氨基-3,6-二脱氧-D-半乳糖（D-Fucp3NAc）残基，这在鲍曼不动杆菌CPS中较为罕见。K89 CPS具有一个→3)-α-D-GalpNAc-(1→3)-β-D-GlcpNAc-(1→主链，带有一个β-D-Glcp-(1→2)-β-D-Fucp3NAc-(1→6)-D-Glcp侧链，该侧链通过α-(1→4)连接到D-GalpNAc。确定了Wzy聚合酶以及KL89基因簇编码的四种糖基转移酶在K89 CPS生物合成中的作用。两种糖基转移酶Gtr121和Gtr122将D-Fucp3NAc与其相邻糖类相连。
