Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.
State Research Center for Applied Microbiology and Biotechnology, City District Serpukhov, Moscow Region, 142279 Obolensk, Russia.
Viruses. 2024 May 13;16(5):771. doi: 10.3390/v16050771.
The genus comprises both environmental and clinically relevant species associated with hospital-acquired infections. Among them, is a critical priority bacterial pathogen, for which the research and development of new strategies for antimicrobial treatment are urgently needed. spp. produce a variety of structurally diverse capsular polysaccharides (CPSs), which surround the bacterial cells with a thick protective layer. These surface structures are primary receptors for capsule-specific bacteriophages, that is, phages carrying tailspikes with CPS-depolymerizing/modifying activities. Phage tailspike proteins (TSPs) exhibit hydrolase, lyase, or esterase activities toward the corresponding CPSs of a certain structure. In this study, the data on all lytic capsule-specific phages infecting spp. with genomes deposited in the NCBI GenBank database by January 2024 were summarized. Among the 149 identified TSPs encoded in the genomes of 143 phages, the capsular specificity (K specificity) of 46 proteins has been experimentally determined or predicted previously. The specificity of 63 TSPs toward CPSs, produced by various K types, was predicted in this study using a bioinformatic analysis. A comprehensive phylogenetic analysis confirmed the prediction and revealed the possibility of the genetic exchange of gene regions corresponding to the CPS-recognizing/degrading parts of different TSPs between morphologically and taxonomically distant groups of capsule-specific phages.
该属包括与医院获得性感染相关的环境和临床相关物种。其中， 是一种关键的优先细菌病原体，迫切需要为其开发新的抗菌治疗策略。 spp. 产生多种结构多样的荚膜多糖 (CPSs)，这些多糖在细菌细胞周围形成一层厚厚的保护层。这些表面结构是荚膜特异性噬菌体的主要受体，即携带具有 CPS 解聚/修饰活性的尾刺的噬菌体。噬菌体尾刺蛋白 (TSP) 对特定结构的相应 CPS 表现出水解酶、裂合酶或酯酶活性。在本研究中，总结了截至 2024 年 1 月在 NCBI GenBank 数据库中提交基因组的感染 spp. 的所有裂解性荚膜特异性噬菌体的数据。在 143 个噬菌体基因组中编码的 149 个鉴定出的 TSP 中，46 个蛋白质的荚膜特异性 (K 特异性) 以前已经通过实验确定或预测。本研究使用生物信息学分析预测了 63 个 TSP 对各种 K 型 CPS 的特异性，综合系统发育分析证实了预测，并揭示了不同 TSP 中识别/降解 CPS 部分的基因区域在形态和分类上差异很大的荚膜特异性噬菌体之间遗传交换的可能性。
