Frijlink H W, Visser J, Drenth B F
J Chromatogr. 1987 Apr 10;415(2):325-33. doi: 10.1016/s0378-4347(00)83224-7.
A rapid and sensitive high-performance liquid chromatographic method for the analysis of beta- and gamma-cyclodextrin in aqueous biological fluids such as plasma, urine, or tissue homogenate is described. The chromatographic system consists of a microBondapak Phenyl column as stationary phase and a mobile phase of water with 10% methanol. After post-column addition of an alkaline solution of phenolphthalein, negative colorimetric detection is used. The elution solvent and post-column reagent were mixed in a capillary tubing of 1.5 m (1.0 mm I.D.). Two methods of sample treatment are given, one for large (1.0 ml) and one for small (0.1 ml) sample volumes. Both methods were shown to be linear and reproducible. The detection limit for beta-cyclodextrin was 1.0 microgram/ml (0.77 nmol/ml). The method was used in the determination of some pharmacokinetic parameters of beta-cyclodextrin in rats after intravenous injection.
本文描述了一种快速灵敏的高效液相色谱法，用于分析血浆、尿液或组织匀浆等水性生物流体中的β-和γ-环糊精。色谱系统由作为固定相的微Bondapak苯基柱和含10%甲醇的水作为流动相组成。柱后加入酚酞碱性溶液后，采用负比色检测。洗脱溶剂和柱后试剂在1.5 m（内径1.0 mm）的毛细管中混合。给出了两种样品处理方法，一种用于大体积（1.0 ml）样品，一种用于小体积（0.1 ml）样品。两种方法均显示出线性和可重复性。β-环糊精的检测限为1.0微克/毫升（0.77纳摩尔/毫升）。该方法用于测定大鼠静脉注射β-环糊精后的一些药代动力学参数。
