Selvidge L A, Eftink M R
Anal Biochem. 1986 May 1;154(2):400-8. doi: 10.1016/0003-2697(86)90005-9.
A spectroscopic displacement method is used to determine association constants of beta-cyclodextrin with compounds that are spectroscopically transparent. These compounds are adamantanecarboxylate and structurally related compounds. Association constants obtained are compared to values obtained by other methods. It is shown that for all types of displacement techniques a distinction must be made between free and total concentrations of ligand in cases of strong binding.
采用光谱位移法测定β-环糊精与光谱透明化合物的缔合常数。这些化合物是金刚烷羧酸盐及结构相关化合物。将所得缔合常数与通过其他方法获得的值进行比较。结果表明，对于所有类型的位移技术，在强结合情况下必须区分配体的游离浓度和总浓度。
