L-三碘甲状腺原氨酸通过一种自分泌因子在一种培养的生长激素分泌细胞系中刺激生长。
L-triiodothyronine stimulates growth by means of an autocrine factor in a cultured growth-hormone-producing cell line.
作者信息
Miller M J, Fels E C, Shapiro L E, Surks M I
出版信息
J Clin Invest. 1987 Jun;79(6):1773-81. doi: 10.1172/JCI113018.
L-Triiodothyronine (T3) stimulates DNA synthesis and replication of cultured GC cells, a T3-responsive growth hormone (GH)-secreting cell line. To determine whether T3 stimulates secretion of an autocrine growth factor, we compared the growth-promoting activity of medium conditioned by T3-stimulated and T3-depleted cells to that of unconditioned medium. Addition of polyclonal rabbit anti-T3 serum to T3-containing media decreased cellular T3 content by 50-70%. In unconditioned medium, anti-T3 serum decreased T3-induced cell growth and GH production by 40-70%. In conditioned medium, anti-T3 serum also effected a 45-70% decrease in induction of GH secretion but did not attenuate the growth-promoting activity. Growth-promoting activity was not detected in medium conditioned by T3-depleted cells. Thus, conditioned medium from T3-containing GC cell cultures contains growth-promoting activity that is independent of T3. Further, the induction of GC cel growth by T3 may occur, at least in part, by induction of an autocrine growth factor.
L-三碘甲状腺原氨酸（T3）可刺激培养的GC细胞（一种对T3有反应的分泌生长激素（GH）的细胞系）的DNA合成和复制。为了确定T3是否刺激自分泌生长因子的分泌，我们将经T3刺激和T3耗尽的细胞所产生的条件培养基的促生长活性与未处理的培养基进行了比较。向含T3的培养基中添加多克隆兔抗T3血清可使细胞内T3含量降低50 - 70%。在未处理的培养基中，抗T3血清使T3诱导的细胞生长和GH产生降低了40 - 70%。在条件培养基中，抗T3血清也使GH分泌诱导降低了45 - 70%，但并未减弱促生长活性。在T3耗尽的细胞所产生的条件培养基中未检测到促生长活性。因此，来自含T3的GC细胞培养物的条件培养基含有与T3无关的促生长活性。此外，T3对GC细胞生长的诱导可能至少部分是通过诱导自分泌生长因子而发生的。
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