通过抗体亲和层析法纯化人干扰素。
Purification of human interferons by antibody affinity chromatography.
作者信息
Berg K, Alacam R, Hamilton R D, Heron I
出版信息
Tex Rep Biol Med. 1977;35:187-92.
PMID:358447
Abstract
Human Leukocyte Interferon, Human Fibroblast Interferon and Namalva-interferon was purified on an antibody affinity colomn up to 2-20 X 10 IFU/mg protein with recoveries in the 80-120% range. The colomn was made by coupling highly absorped anti-leukocyte interferon immunoglobulins to Sepharose 4B. The absorption was performed by means of crude human leukocyte interferon covalently bound to Sepharose 4B.
摘要
人白细胞干扰素、人成纤维细胞干扰素和纳马伐干扰素在抗体亲和柱上进行纯化，纯化后的蛋白浓度可达2 - 20×10国际单位/毫克，回收率在80% - 120%之间。该柱是通过将高度吸收的抗白细胞干扰素免疫球蛋白偶联到琼脂糖4B上制成的。吸收过程采用与琼脂糖4B共价结合的粗制人白细胞干扰素来进行。
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