Berg K, Ogburn C A, Paucker K, Mogensen K E, Cantell K
J Immunol. 1975 Feb;114(2 Pt 1):640-4.
Interferons produced in human peripheral leukocytes (LE) and foreskin fibroblast (FS-4) cells were subjected to affinity chromatography on Sepharose-bound globulins from rabbits immunized with these interferons. Anti-LE interferon sera neutralized both interferons, but titers against FS-4 interferon were consistently lower than those against LE interferon. Anti-FS-4 interferon sera neutralized only FS-4 but not LE interferon. Accordingly, affinity columns constructed with anti-FS-4 globulin excluded LE but not FS-4 interferon, whereas those prepared with anti-LE interferon globulin bound and eluted both LE and FS-4 interferons. Purification of native interferons of both types on anti-LE interferon-Sepharose ranged from 680- to 3,600-fold and recoveries from 72 to 126%. Specific activities of eluate pools varied from 4 to 30 times 10-6 reference (B, 69/19) units per milligram protien.
对在人外周血白细胞（LE）和包皮成纤维细胞（FS - 4）中产生的干扰素进行亲和层析，所用的琼脂糖结合球蛋白来自用这些干扰素免疫的兔子。抗LE干扰素血清能中和这两种干扰素，但针对FS - 4干扰素的效价始终低于针对LE干扰素的效价。抗FS - 4干扰素血清仅能中和FS - 4干扰素，而不能中和LE干扰素。因此，用抗FS - 4球蛋白构建的亲和柱能排除LE干扰素，但不能排除FS - 4干扰素，而用抗LE干扰素球蛋白制备的亲和柱能结合并洗脱LE和FS - 4两种干扰素。在抗LE干扰素 - 琼脂糖上对两种天然干扰素的纯化倍数在680至3600倍之间，回收率在72%至126%之间。洗脱液池的比活性为每毫克蛋白质4至30×10⁻⁶参考（B，69/19）单位。
