Alonso-Nieves Ana Laura, Salazar-Vidal M Nancy, Torres-Rodríguez J Vladimir, Pérez-Vázquez Leonardo M, Massange-Sánchez Julio A, Gillmor C Stewart, Sawers Ruairidh J H
Langebio, Unidad de Genómica Avanzada Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN) Irapuato Mexico.
Department of Evolution and Ecology University of California, Davis Davis California USA.
Plant Direct. 2022 Jul 12;6(7):e416. doi: 10.1002/pld3.416. eCollection 2022 Jul.
Plant PHO1 proteins play a central role in the translocation and sensing of inorganic phosphate. The maize ( ssp. ) genome encodes two co-orthologs of the gene, designated and . Here, we report the characterization of the transposon footprint allele , which we refer to hereafter as . The allele is a stable derivative formed by excision of an transposable element from the gene. The allele contains an 8-bp insertion at the point of transposon excision that disrupts the reading frame and is predicted to generate a premature translational stop. We show that the allele is linked to a dosage-dependent reduction in transcript accumulation and a mild reduction in seedling growth. Characterization of shoot and root transcriptomes under full nutrient, low nitrogen, low phosphorus, and combined low nitrogen and low phosphorus conditions identified 1100 differentially expressed genes between wild-type plants and plants carrying the mutation. Of these 1100 genes, 966 were upregulated in plants carrying , indicating the wild-type PHO1;2a to predominantly impact negative gene regulation. Gene set enrichment analysis of the -misregulated genes revealed associations with phytohormone signaling and the phosphate starvation response. In roots, differential expression was broadly consistent across all nutrient conditions. In leaves, differential expression was largely specific to low phosphorus and combined low nitrogen and low phosphorus conditions. Of 276 genes upregulated in the leaves of mutants in the low phosphorus condition, 153 were themselves induced in wild-type plants with respect to the full nutrient condition. Our observations suggest that functions in the fine-tuning of the transcriptional response to phosphate starvation through maintenance and/or sensing of plant phosphate status.
植物PHO1蛋白在无机磷酸盐的转运和感知中起核心作用。玉米（亚种）基因组编码该基因的两个直系同源基因，命名为 和 。在此，我们报道了转座子足迹等位基因 的特征，此后我们将其称为 。 等位基因是由 转座元件从 基因中切除形成的稳定衍生物。 等位基因在转座子切除位点含有一个8碱基对的插入，破坏了阅读框，并预计会产生提前的翻译终止。我们表明， 等位基因与 转录本积累的剂量依赖性降低以及幼苗生长的轻度降低有关。在全营养、低氮、低磷以及低氮和低磷组合条件下对地上部和根部转录组的表征确定了野生型植物和携带 突变的植物之间1100个差异表达基因。在这1100个基因中，966个在携带 的植物中上调，表明野生型PHO1;2a主要影响负基因调控。对 失调基因的基因集富集分析揭示了与植物激素信号传导和磷饥饿反应的关联。在根中，所有营养条件下的差异表达大致一致。在叶中，差异表达在很大程度上特定于低磷以及低氮和低磷组合条件。在低磷条件下 突变体叶片中上调的276个基因中，相对于全营养条件，其中153个在野生型植物中本身也被诱导。我们的观察结果表明， 通过维持和/或感知植物磷状态在对磷饥饿的转录反应的微调中发挥作用。
