Liu Qian, Fu Chao, Yang Hai, Zhou Chuanshe, Kang Jinhe, Chen Liang, Kong Zhiwei, Tan Zhiliang, Tang Shaoxun
College of Life Science and Environment Hengyang Normal University Hengyang China.
Key Laboratory for Agro-Ecological Processes in Subtropical Region National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process Institute of Subtropical Agriculture Chinese Academy of Sciences Changsha China.
Food Sci Nutr. 2022 Apr 1;10(7):2400-2407. doi: 10.1002/fsn3.2848. eCollection 2022 Jul.
The study investigated the effect of alfalfa hay substituted with ramie silage on the expression of apoptotic genes in the gastrointestinal tract of goats. Thirty-two goats were randomly allocated into four groups, in which the alfalfa was substituted with ramie at 0%, 35%, 75%, and 100% levels, respectively. In the rumen, the mRNA expression of Bax was significantly up-regulated ( = .0007) when alfalfa was 100% substituted by ramie; the mRNA expression of Bcl-2/Bax was significantly down-regulated ( = .02) when alfalfa was 100% substituted by ramie compared with the 75% substituted treatment; the protein expression of Bcl-xl was significantly down-regulated ( = .03) when alfalfa was 100% substituted by ramie compared with 35% and 75% substituted treatments, respectively. In the jejunum, the mRNA expression of p53 was significantly up-regulated ( = .01) when alfalfa was 100% substituted by ramie compared with 0% and 35% substituted treatments; the protein expression of p53 was significantly up-regulated ( = .001) when alfalfa was 35% substituted by ramie compared with 0% and 75% substituted treatments. However, the activity of Caspase-3 was not affected by different substituting levels of ramie in the rumen and jejunum of goats ( > .05). In conclusion, ramie with high substitution had strong antinutritional effect, which might promote the apoptosis in the gastrointestinal tract of goats in a caspase-independent manner, thus affecting the growth and development of goat. It was suggested that ramie should not replace alfalfa more than 35% in the process of goat feeding.
本研究调查了用苎麻青贮料替代苜蓿干草对山羊胃肠道凋亡基因表达的影响。32只山羊被随机分为四组，苜蓿分别被苎麻以0%、35%、75%和100%的水平替代。在瘤胃中，当苜蓿被苎麻100%替代时，Bax的mRNA表达显著上调（P = 0.0007）；与75%替代处理相比，当苜蓿被苎麻100%替代时，Bcl-2/Bax的mRNA表达显著下调（P = 0.02）；与35%和75%替代处理相比，当苜蓿被苎麻100%替代时，Bcl-xl的蛋白表达显著下调（P = 0.03）。在空肠中，与0%和35%替代处理相比，当苜蓿被苎麻100%替代时，p53的mRNA表达显著上调（P = 0.01）；与0%和75%替代处理相比，当苜蓿被苎麻35%替代时，p53的蛋白表达显著上调（P = 0.001）。然而，山羊瘤胃和空肠中不同苎麻替代水平对Caspase-3活性没有影响（P > 0.05）。总之，高替代水平的苎麻具有较强的抗营养作用，可能以不依赖半胱天冬酶的方式促进山羊胃肠道细胞凋亡，从而影响山羊的生长发育。建议在山羊饲养过程中，苎麻替代苜蓿的比例不应超过35%。
